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Simultaneous Quantification of Uronic Acid, Amino Sugar, and Neutral Sugar in the Acidic Polysaccharides Extracted from the Roots of Angelica sinensis (Oliv.) Diels by HPLC

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Abstract

High-performance liquid chromatography (HPLC) is routinely used to analyze monosaccharide composition. This is the first report describing the simultaneous determination of uronic acid, amino sugar, and neutral sugar in the polysaccharide from Angelica sinensis using pre-column derivatization. We were able to analyze the monosaccharide composition of acidic Angelica polysaccharides (AAPS) using this method. Ten different monosaccharides, namely d-mannose, d-glucosamine, l-rhamnose, d-galactosamine, d-glucuronic acid, d-galacturonic acid, d-glucose, d-galactose, l-arabinose, and l-fucose were made into pre-column derivatives to serve as the standard for AAPS analysis. Samples were separated on a Hypersil BDS C18 column (4.6 mm × 250 mm, i.d.) and detected by UV detection at 250 nm wavelength. The mobile phase was composed of aqueous ammonium acetate buffer (100 mM, pH 5.0), acetonitrile, and tetrahydrofuran in the ratio of 81:17:2. Analytes were performed at 25 °C with a flow rate of 1.0 ml/min. Our study found that AAPS is composed of d-mannose, d-glucosamine, l-rhamnose, d-galactosamine, d-glucuronic acid, d-galacturonic acid, and d-glucose in a molar ratio of 1.5:14.1:1.7:1.6:2.0:2.3:1.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (81173513, 30701081, and 81473329).

Conflict of Interest

Wei-Yan Li, Ping Li, Xiao-Qiang Li, Hai Huang, Hanwei Yan, Ya Zhang and Wei Cao declare that they have no conflict of interest.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Department of Natural Medicine and Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, Xi’an, 710032, China

    Wei-Yan Li, Ping Li, Hai Huang, Hanwei Yan, Ya Zhang & Wei Cao

  2. Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an, 710032, China

    Xiao-Qiang Li

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  1. Wei-Yan Li
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  2. Ping Li
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  3. Xiao-Qiang Li
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  4. Hai Huang
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  5. Hanwei Yan
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  6. Ya Zhang
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  7. Wei Cao
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Correspondence to Wei Cao.

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Li, WY., Li, P., Li, XQ. et al. Simultaneous Quantification of Uronic Acid, Amino Sugar, and Neutral Sugar in the Acidic Polysaccharides Extracted from the Roots of Angelica sinensis (Oliv.) Diels by HPLC. Food Anal. Methods 8, 2087–2093 (2015). https://doi.org/10.1007/s12161-015-0096-8

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  • DOI: https://doi.org/10.1007/s12161-015-0096-8

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