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Evaluation of Two Immunomagnetic Separation Techniques for the Detection and Recovery of Escherichia coli O157:H7 from Finished Composts

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Abstract

Two rapid immunomagnetic separation (IMS) protocols were evaluated to recover inoculated Escherichia coli O157:H7 (1–2 log colony-forming unit (CFU)/g) from 30 different commercial, finished compost samples. Both protocols detected E. coli O157:H7 in compost samples; PCR techniques required the removal of inhibitors to reduce possibility of a false negative result.

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Funding

Funding was provided by the Center for Produce Safety grant “Validation of testing methods for the detection and quantification of Escherichia coli O157:H7, Salmonella spp., fecal coliforms and non-pathogenic Escherichia coli in compost” and the USDA-ARS “Microbial Ecology & Safety of Fresh on-Farm Organically Grown Produce” project.

Conflict of Interest

Russell Reynnells declares that he has no conflict of interest. Mary Theresa Callahan declares that she has no conflict of interest. Eric T. Handy declares that he has no conflict of interest. Cheryl Roberts declares that she has no conflict of interest. Gary Felton declares that he has no conflict of interest. David T. Ingram declares that he has no conflict of interest. Patricia D. Millner has received research funding from the Center for Produce Safety. Manan Sharma has received research funding from the Center for Produce Safety. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. United States Department of Agriculture, Agricultural Research Service, Environmental Microbial and Food Safety Laboratory, Henry A. Wallace Beltsville Area Research Center, Bldg 201, Rm 103, 10300 Baltimore Ave, Beltsville, MD, 20705, USA

    Russell Reynnells, Mary Theresa Callahan, Eric T. Handy, Cheryl Roberts, Patricia D. Millner & Manan Sharma

  2. Environmental Science and Technology Department, 1433 Animal Science/Agricultural Engineering, University of Maryland, College Park, MD, 20742-2315, USA

    Gary Felton

  3. Center for Food Safety and Applied Nutrition, Food and Drug Administration, 5100 Paint Branch Parkway, College Park, MD, 20740, USA

    David T. Ingram

Authors
  1. Russell Reynnells
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  2. Mary Theresa Callahan
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  3. Eric T. Handy
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  4. Cheryl Roberts
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  5. Gary Felton
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  6. David T. Ingram
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  7. Patricia D. Millner
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  8. Manan Sharma
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Corresponding author

Correspondence to Manan Sharma.

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Reynnells, R., Callahan, M.T., Handy, E.T. et al. Evaluation of Two Immunomagnetic Separation Techniques for the Detection and Recovery of Escherichia coli O157:H7 from Finished Composts. Food Anal. Methods 8, 1812–1814 (2015). https://doi.org/10.1007/s12161-014-0068-4

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  • DOI: https://doi.org/10.1007/s12161-014-0068-4

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