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A Novel Electrochemical Biosensor Based on a Modified Gold Electrode for Hydrogen Peroxide Determination in Different Beverage Samples

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Abstract

In the present work, an electrochemical biosensor is constructed based on a Nafion nano composite polymer, toluidine blue (TB) and catalase enzyme-modified gold electrode (AuE). The TB molecules were strongly adsorbed on the Nafion/AuE. The electrochemical properties of this biosensor were examined. Cyclic voltammetry was used at various scan rates to investigate the redox properties of the Nafion and TB-modified AuE (Nafion/TB/AuE). The electron transfer coefficient, α, and the electron transfer rate constant, k s, were found to be 0.48 and 12.1 ± 0.3 s−1 in pH 7.0, respectively. The Nafion, TB, and catalase-modified AuE (Nafion/TB/catalase/AuE) exhibited excellent electrocatalytic response to the reduction of hydrogen peroxide (H2O2). Using cyclic voltammetry, kinetic parameters such as electron transfer coefficient, α, and heterogeneous rate constant, k’, were determined for the reduction of H2O2 at this biosensor surface. Differential pulse voltammetry exhibited two linear dynamic ranges and a detection limit of 0.25 μM for H2O2.

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Conflict of Interest

Navid Nasirizadeh has no conflict of interest. Saeedeh hajihosseini has no conflict of interest. Zahra Shekari has no conflict of interest. Masoud Ghaani has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Scientific Society of Nanotechnology, Yazd Branch, Islamic Azad University, Yazd, Iran

    Navid Nasirizadeh, Saeedeh hajihosseini & Zahra Shekari

  2. Department of Textile Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran

    Navid Nasirizadeh

  3. Department of food science & technology, Science and Research Branch, Islamic Azad University, Yazd, Iran

    Masoud Ghaani

Authors
  1. Navid Nasirizadeh
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  2. Saeedeh hajihosseini
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  3. Zahra Shekari
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  4. Masoud Ghaani
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Correspondence to Navid Nasirizadeh.

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Nasirizadeh, N., hajihosseini, S., Shekari, Z. et al. A Novel Electrochemical Biosensor Based on a Modified Gold Electrode for Hydrogen Peroxide Determination in Different Beverage Samples. Food Anal. Methods 8, 1546–1555 (2015). https://doi.org/10.1007/s12161-014-0041-2

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  • DOI: https://doi.org/10.1007/s12161-014-0041-2

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