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Quantification of Se-Methylselenocysteine and Its γ-Glutamyl Derivative from Naturally Se-Enriched Green Bean (Phaseolus vulgaris vulgaris) After HPLC-ESI-TOF-MS and Orbitrap MSn-Based Identification

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Abstract

Orthogonal liquid chromatographic (ion exchange, reversed phase, and ion pairing) and mass spectrometric [electrospray ionization (ESI)-TOF-MS, ESI-Orbitrap MS, and inductively coupled plasma mass spectrometry (ICP-MS)] methods were addressed to identify and quantify selenium species from a naturally Se-enriched green bean (Phaseolus vulgaris vulgaris) sample after proteolytic digestion. While selenomethionine (10.1 mg/kg as Se) and selenate (9.5 mg/kg as Se) could be quantified in a straightforward way by anion exchange LC-ICP-MS technique, a multistep purification protocol was required to identify Se-methylselenocysteine and γ-glutamyl-Se-methylselenocysteine in an unambiguous way prior to quantification by using either in-source fragmentation (LC-ESI-TOF-MS) or collision-induced dissociation (LC-ESI-Orbitrap MS). Finally, Se-methylselenocysteine (2.6 mg/kg as Se) and γ-glutamyl-Se-methylselenocysteine (1.2 mg/kg as Se) could contribute to the overall selenium recovery of 72 %. This sample is the first of the Faboideae subfamily and Phaseolus ssp. to be speciated to such an extent for selenium including γ-glutamyl-Se-methylselenocysteine, a highly potential selenium species, which makes this bean material an ideal candidate for functional food purposes.

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Acknowledgments

This project was supported by the National Science Foundation of China (grant no. 40971287) and the “Strategic Priority Research Program—Climate change: carbon budget and related issues” of the Chinese Academy of Sciences (grant no. XDA05010105), and the 12th 5-year plan project of State Key Laboratory of Ore-Deposit Geochemistry, Chinese Academy of Sciences (SKLODG-ZY125-08). M. Dernovics acknowledges the financial support from the Magyary Zoltán Higher Educational Public Foundation. The authors also acknowledge the TÁMOP grants, nos. 4.2.1./B-09/1/KMR-2010-0005 and 4.2.2/B-10/1-2010-0023, and the grant ref. HH-2008-0018 from the Spanish MEC.

Conflict of Interest

Shuxun Shao declares that he has no conflict of interest. Xiubo Mi declares that he has no conflict of interest. Laurent Ouerdane declares that he has no conflict of interest. Ryszard Lobinski declares that he has no conflict of interest. Juan Francisco García-Reyes declares that he has no conflict of interest. Antonio Molina-Díaz declares that he has no conflict of interest. Andrea Vass declares that she has no conflict of interest. Mihály Dernovics declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. State Key Laboratory of Ore Deposit Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, 46 Guanshui Road, Guiyang, 550002, China

    Shuxun Shao & Xiubo Mi

  2. College of Earth Science, University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing, 100049, China

    Xiubo Mi

  3. Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, UMR 5254, CNRS/UPPA, Hélioparc, 2 av. Pierre Angot, 64053, Pau, France

    Laurent Ouerdane & Ryszard Lobinski

  4. Analytical Chemistry Research Group, Department of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas, Edif B3, 23071, Jaén, Spain

    Juan Francisco García-Reyes & Antonio Molina-Díaz

  5. Department of Applied Chemistry, Corvinus University of Hungary, Villányi út 29-33, 1118, Budapest, Hungary

    Andrea Vass & Mihály Dernovics

  6. Department of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664, Warsaw, Poland

    Ryszard Lobinski

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  1. Shuxun Shao
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Shao, S., Mi, X., Ouerdane, L. et al. Quantification of Se-Methylselenocysteine and Its γ-Glutamyl Derivative from Naturally Se-Enriched Green Bean (Phaseolus vulgaris vulgaris) After HPLC-ESI-TOF-MS and Orbitrap MSn-Based Identification. Food Anal. Methods 7, 1147–1157 (2014). https://doi.org/10.1007/s12161-013-9728-z

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