Article

BioEnergy Research

, Volume 6, Issue 1, pp 292-299

Ethanol Production from High-Solid SSCF of Alkaline-Pretreated Corncob Using Recombinant Zymomonas mobilis CP4

  • Rongxin SuAffiliated withState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University
  • , Yuanyuan MaAffiliated withBiomass Conversion Laboratory of Tianjin University R&D Center for Petrochemical Technology, Tianjin University
  • , Wei QiAffiliated withState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University
  • , Mingjia ZhangAffiliated withState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University
  • , Fang WangAffiliated withState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University
  • , Ruoyu DuAffiliated withState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University
  • , Jifeng YangAffiliated withState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University
  • , Minhua ZhangAffiliated withBiomass Conversion Laboratory of Tianjin University R&D Center for Petrochemical Technology, Tianjin University Email author 
  • , Zhimin HeAffiliated withState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University Email author 

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Abstract

In this work, Zymomonas mobilis was genetically improved for pentose utilization to increase the final ethanol concentration. It showed good fermentation ability on both soluble sugar mixture and lignocellulose. Nearly all the glucose and xylose in sugar mixture can be consumed, corresponding to 86 % of theoretic ethanol yield. Simultaneous saccharification and co-fermentation (SSCF) of NaOH-pretreated corncob was then carried out in a high dry matter (DM) loading of 15–25 w/v%. At the DM loading of 15 %, the suitable operating conditions were determined, i.e., Z. mobilis loading of 0.30 g dry weight/L at 30 °C (pH 5.5), under which the ethanol concentration reached 49.2 g/L. Higher final ethanol concentrations were obtained when SSCF was operated at the fed-batch mode. Several amounts of substrate (1 % to 10 %) were added, and the highest final ethanol concentration (60.5 g/L) was obtained at 10 % DM addition.

Keywords

Simultaneous saccharification and co-fermentation Recombinant Zymomonas mobilis Corncob Bioethanol Xylose fermentation Lignocellulose Cellulose High solids