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FluoroNanogold: an important probe for correlative microscopy

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  • Published:
Journal of Chemical Biology

Abstract

Correlative microscopy is a powerful imaging approach that refers to observing the same exact structures within a specimen by two or more imaging modalities. In biological samples, this typically means examining the same sub-cellular feature with different imaging methods. Correlative microscopy is not restricted to the domains of fluorescence microscopy and electron microscopy; however, currently, most correlative microscopy studies combine these two methods, and in this review, we will focus on the use of fluorescence and electron microscopy. Successful correlative fluorescence and electron microscopy requires probes, or reporter systems, from which useful information can be obtained with each of the imaging modalities employed. The bi-functional immunolabeling reagent, FluoroNanogold, is one such probe that provides robust signals in both fluorescence and electron microscopy. It consists of a gold cluster compound that is visualized by electron microscopy and a covalently attached fluorophore that is visualized by fluorescence microscopy. FluoroNanogold has been an extremely useful labeling reagent in correlative microscopy studies. In this report, we present an overview of research using this unique probe.

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Acknowledgments

This work was supported in part by NIH grant HD058084 (JMR). We wish to acknowledge the staff in the Campus Microscopy and Imaging Facility at the Ohio State University Wexner Medical Center for assistance in collecting some of the data presented in this review.

Conflict of interest

JFH is the President of and RDP is employed by Nanoprobes, Inc.

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Authors and Affiliations

  1. Department of Molecular Anatomy, Nippon Medical School, Tokyo, Japan

    Toshihiro Takizawa

  2. Nanoprobes, Incorporated, 95 Horseblock Road, Unit 1, Yaphank, NY, 11980-9710, USA

    Richard D. Powell & James F. Hainfeld

  3. Department of Physiology and Cell Biology, Ohio State University, Columbus, OH, 43210, USA

    John M. Robinson

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  1. Toshihiro Takizawa
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Correspondence to Richard D. Powell.

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Takizawa, T., Powell, R.D., Hainfeld, J.F. et al. FluoroNanogold: an important probe for correlative microscopy. J Chem Biol 8, 129–142 (2015). https://doi.org/10.1007/s12154-015-0145-1

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