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Backbone and side chain NMR assignments for the ribosome assembly factor Nop6 from Saccharomyces cerevisiae

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Abstract

The Saccharomyces cerevisiae Nop6 protein is involved in the maturation of the small ribosomal subunit. It contains a central RNA binding domain and a predicted C-terminal coiled-coil domain. Here we report the almost complete (>90 %) 1H,13C,15N backbone and side chain NMR assignment of a 15 kDa Nop6 construct comprising the RNA binding and coiled-coil domains.

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References

  • Buchhaupt M, Kötter P, Entian K-D (2007) Mutations in the nucleolar proteins Tma23 and Nop6 suppress the malfunction of the Nep1 protein. FEMS Yeast Res 7:771–781

    Article  Google Scholar 

  • Eghbalnia HR, Wang L, Bahrami A, Assadi A, Markley JL (2005) Protein energetic conformational analysis from NMR chemical shifts (PECAN) and its use in determining secondary structural elements. J Biomol NMR 32:71–81

    Article  Google Scholar 

  • García-Gómez JJ, Babiano R, Lebaron S, Froment C, Monsarrat B, Henry Y, de la Cruz J (2011) Nop6, a component of 90S pre-ribosomal particles, is required for 40S ribosomal subunit biogenesis in Saccharomyces cerevisiae. RNA Biol 8:112–124

    Article  Google Scholar 

  • Henras AK, Soudet J, Gerus M, Lebaron S, Caizergues-Ferrer M, Mougin A, Henry Y (2008) The post-transcriptional steps of eukaryotic ribosome biogenesis. Cell Mol Life Sci 65:2334–2359

    Article  Google Scholar 

  • Ishida T, Kinoshita K (2008) Prediction of disordered regions in proteins based on the meta approach. Bioinformatics 24:1344–1348

    Article  Google Scholar 

  • Keller R (2004) The computer aided resonance assignment tutorial. CANTINA Verlag, Goldau

  • Lemay V, Hossain A, Osheim YN, Beyer NL, Dragon F (2011) Identification of novel proteins associated with yeast snR30 small nucleolar RNA. Nucleic Acids Res 39:9659–9670

    Article  Google Scholar 

  • Malakhov M, Mattern M, Malakhova O, Drinker M, Weeks S, Butt T (2004) SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins. J Struct Funct Genomics 5:75–86

    Article  Google Scholar 

  • Sattler M, Schleucher J, Griesinger C (1999) Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients. Prog Nucl Magn Reson Spectrosc 34:93–158

    Article  Google Scholar 

  • Taylor AB, Meyer B, Leal BZ, Kötter P, Schirf V, Demeler B, Hart PJ, Entian KD, Wöhnert J (2008) The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site. Nucleic Acids Res 36:1542–1554

    Article  Google Scholar 

  • Wurm JP, Meyer B, Bahr U, Held M, Frolow O, Kötter P, Engels JW, Heckel A, Karas M, Entian K-D, Wöhnert J (2010) The ribosome assembly factor Nep1 responsible for Bowen–Conradi syndrome is a pseudouridine-N1-specific methyltransferase. Nucleic Acids Res 38:2387–2398

    Article  Google Scholar 

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Acknowledgments

We are grateful to Dr. Elke Duchardt-Ferner for helpful discussions. This project was supported by an Aventis Foundation Professorship (to J.W.), the Center of Biomolecular Magnetic Resonance, Johann-Wolfgang-Goethe-University Frankfurt, and the Deutsche Forschungsgemeinschaft (DFG) through the SFB 902 ‘Molecular Principles of RNA-based Regulation’.

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Authors and Affiliations

  1. Institut für Molekulare Biowissenschaften, Johann-Wolfgang-Goethe-Universität Frankfurt/M, Max-von-Laue-Str. 9, 60438, Frankfurt, Germany

    Jan Philip Wurm, Anatoli Lioutikov, Peter Kötter, Karl-Dieter Entian & Jens Wöhnert

  2. Center for Biomolecular Magnetic Resonance (BMRZ), Johann-Wolfgang-Goethe-Universität, 60438, Frankfurt, Germany

    Jan Philip Wurm & Jens Wöhnert

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  1. Jan Philip Wurm
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  2. Anatoli Lioutikov
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  3. Peter Kötter
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  4. Karl-Dieter Entian
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  5. Jens Wöhnert
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Correspondence to Jens Wöhnert.

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Wurm, J.P., Lioutikov, A., Kötter, P. et al. Backbone and side chain NMR assignments for the ribosome assembly factor Nop6 from Saccharomyces cerevisiae . Biomol NMR Assign 8, 345–348 (2014). https://doi.org/10.1007/s12104-013-9514-2

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  • DOI: https://doi.org/10.1007/s12104-013-9514-2

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