Article

Biomolecular NMR Assignments

, Volume 7, Issue 2, pp 321-324

Open Access This content is freely available online to anyone, anywhere at any time.

1H, 13C, and 15N backbone and side chain resonance assignments of the C-terminal DNA binding and dimerization domain of v-Myc

  • Gönül KızılsavaşAffiliated withMax F. Perutz Laboratories, Department of Structural and Computational Biology, University of Vienna
  • , Saurabh SaxenaAffiliated withFaculty of Chemistry, University of Warsaw
  • , Szymon ŻerkoAffiliated withFaculty of Chemistry, University of Warsaw
  • , Wiktor KoźmińskiAffiliated withFaculty of Chemistry, University of Warsaw
  • , Klaus BisterAffiliated withInstitute of Biochemistry, Center for Molecular Biosciences (CMBI), University of Innsbruck
  • , Robert KonratAffiliated withMax F. Perutz Laboratories, Department of Structural and Computational Biology, University of Vienna Email author 

Abstract

The oncogenic transcription factor Myc is one of the most interesting members of the basic-helix-loop-helix-zipper (bHLHZip) protein family. Deregulation of Myc via gene amplification, chromosomal translocation or other mechanisms lead to tumorigenesis including Burkitt lymphoma, multiple myeloma, and many other malignancies. The oncogene myc is a highly potent transforming gene and capable to transform various cell types in vivo and in vitro. Its oncogenic activity initialized by deregulated expression leads to a shift of the equilibrium in the Myc/Max/Mad network towards Myc/Max complexes. The Myc/Max heterodimerization is a prerequisite for transcriptional functionality of Myc. Primarily, we are focusing on the apo-state of the C-terminal domain of v-Myc, the retroviral homolog of human c-Myc. Based on multi-dimensional NMR measurements v-Myc appears to be neither a fully structured nor a completely unstructured protein. The bHLHZip domain of v-Myc does not exist as a random coil but exhibits partially pre-formed α-helical regions in its apo-state. In order to elucidate the structural propensities of Myc in more detail, the backbone and side-chain assignments obtained here for apo-Myc are a crucial prerequisite for further NMR measurements.

Keywords

Myc Oncogene Transcription factor Random sampling Intrinsically disordered protein