Abstract
Kelch-like ECH-associated Protein 1 (Keap1) is a multi-domain protein that functions as an inhibitor of the transcription factor nuclear factor E2-related factor 2 (Nrf2) in the cellular response to oxidative stress. Under normal conditions, Keap1 binds to Nrf2 via its C-terminal Kelch domain and the interaction ultimately leads to the ubiquitin-dependent degradation of Nrf2. It has been proposed that designing molecules to selectively disrupt the Keap1–Nrf2 interaction can be a potential therapeutic approach for enhancing the expression of cytoprotective genes. Here, we reported the 1H, 13C, and 15N backbone chemical shift assignments of the Kelch domain of mouse Keap1. Further, unlabeled Nrf2 peptide containing the Kelch-binding motif was added to the 15N-labeled Kelch sample. 1H–15N HSQC spectra of the protein in the absence and presence of an equimolar concentration of the Nrf2 peptide were presented. A significant number of resonance signals were shifted upon addition of the peptide, confirming the protein–peptide interaction. The results here will not just facilitate the further studies of the binding between Keap1 and Nrf2, it will also be valuable for probing interactions between the Kelch domain and small molecules, as well as a growing list of protein targets that have been identified recently.
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Acknowledgments
This work was supported by an Operating Grant (MOP No. 74679) from the Canadian Institutes of Health Research (CIHR). We are grateful for the use of the NMR facility at National University of Singapore. We would also like to thank the Canadian National High Field NMR Centre (NANUC) for their assistance and use of the facilities. Operation of NANUC is funded by the Natural Science and Engineering Research Council of Canada, and the University of Alberta.
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Cino, E., Fan, J., Yang, D. et al. 1H, 15N and 13C backbone resonance assignments of the Kelch domain of mouse Keap1. Biomol NMR Assign 7, 149–153 (2013). https://doi.org/10.1007/s12104-012-9398-6
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DOI: https://doi.org/10.1007/s12104-012-9398-6