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Backbone and sidechain 1H, 15N and 13C assignments of Tyrosine Phosphatase related to Biofilm formation A (TpbA) of Pseudomonas aeruginosa

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Abstract

The backbone and side chain resonance assignments of the Tyrosine Phosphatase related to Biofilm formation A (TpbA) of Pseudomonas aeruginosa have been determined based on triple-resonance experiments using uniformly [13C,15N]-labeled protein. This assignment is the first step towards the determination of the 3-dimensional structure of TpbA.

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Acknowledgments

The authors thank Dr. David A. Critton for help at early stages of the project. This work was supported by NSF CAREER (MCB0952550) to R. P and NIH (GM089999) to T. K. W. 800 MHz NMR data were recorded at Brandeis University (NIH S10-RR017269).

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Authors and Affiliations

  1. Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, 02903, USA

    Dorothy Koveal & Rebecca Page

  2. Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence, RI, 02903, USA

    Thusitha B. Jayasundera & Wolfgang Peti

  3. Departments of Chemical Engineering and Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, 16802, USA

    Thomas K. Wood

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  1. Dorothy Koveal
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  2. Thusitha B. Jayasundera
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  3. Thomas K. Wood
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  4. Wolfgang Peti
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  5. Rebecca Page
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Correspondence to Wolfgang Peti or Rebecca Page.

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Koveal, D., Jayasundera, T.B., Wood, T.K. et al. Backbone and sidechain 1H, 15N and 13C assignments of Tyrosine Phosphatase related to Biofilm formation A (TpbA) of Pseudomonas aeruginosa . Biomol NMR Assign 7, 57–59 (2013). https://doi.org/10.1007/s12104-012-9376-z

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  • DOI: https://doi.org/10.1007/s12104-012-9376-z

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