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Backbone and side chain 1H, 13C, and 15N NMR assignments for the organic hydroperoxide resistance protein (Ohr) from Burkholderia pseudomallei

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Abstract

Burkholderia pseudomallei is a NIAID Category B microorganism responsible for melioidosis. Here we report backbone and side chain NMR assignments for the 139-residue, homodimeric, organic hydroperoxide resistance protein (Ohr) from this organism.

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Acknowledgments

This research was funded by NIAID under Federal Contract no. HHSN272200700057C and performed primarily at the W.R. Wiley Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by US Department of Energy’s Office of Biological and Environmental Research (BER) program located at Pacific Northwest National Laboratory (PNNL). PNNL is operated for the US Department of Energy by Battelle. The authors also thank Dr. Sam Miller (University of Washington) for kindly providing the B. pseudomallei strain 1710b (Q3JK82) genomic DNA and the entire SSGCID team (especially Dr. Isabelle Phan (SBRI) for bioinformatics assistance).

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Correspondence to Garry W. Buchko.

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Buchko, G.W., Hewitt, S.N., Napuli, A.J. et al. Backbone and side chain 1H, 13C, and 15N NMR assignments for the organic hydroperoxide resistance protein (Ohr) from Burkholderia pseudomallei . Biomol NMR Assign 3, 163–166 (2009). https://doi.org/10.1007/s12104-009-9165-5

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