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Dietary Saccharomyces cerevisiae Boosts Growth and Immunity of IMC Labeo rohita (Ham.) Juveniles

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Abstract

The study attempted to confirm the efficacy of Saccharomyces cerevisiae as a probiotic in augmenting the overall wellbeing and disease resistance in the Indian Major Carp, rohu (Labeo rohita) juveniles. The growth rate, nutritional quality and immunity of L. rohita fry were studied for 60 days fed with four isocaloric and isonitrogenous diets supplemented with 0.50 % (L1), 0.75 % (L2) and 1.00 % (L3) lyophilized whole yeast (S. cerevisiae) cells. L3-fed fish registered significantly better in wellbeing parameters such as growth, RNA:DNA, lower feed conversion and higher protein efficiency ratios. High intestinal enzyme (protease and α-amylase) activities, high liver serum GOT and GPT activities, and better non-specific immune responses were also demonstrated by them. The blood parameters hemoglobin, total erythrocyte and leukocyte counts, corpuscular volume, corpuscular hemoglobin, and cell hemoglobin concentration were also encouraging. Challenged with Aeromonas hydrophila AH2 (hourly exposure to 105 and 107 CFUs/ml strengths with a week interval) by bath exposure, highest survival percentage (96.66 %) was observed in L3-fed fish, whereas only 30 % in the control, after 10 days.

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Notes

  1. All the values are provided on per liter basis; the final pH was adjusted to 5.5.

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Acknowledgments

In terms of materials and facilities, the support and cooperation received from the CIFA, Bhubaneswar for the various wet-lab analyses, the IMMT, Bhubaneswar for the scanning electron microscopy facility, and the IMTECH, Chandigarh for the pathogenic strain used in the study are acknowledged.

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Author notes

  1. Biplab Sarkar

    Present address: National Institute of Abiotic Stress Management, Malegaon, Pune, Maharashtra, India

Authors and Affiliations

  1. Biostadt India Limited, 6th Floor, A Wing, Poonam Chambers, Dr. AB Road, Worli, Mumbai, 400 018, Maharashtra, India

    Partha Bandyopadhyay

  2. School of Biotechnology, Campus-11, KIIT University, Bhubaneswar, 751 024, Odisha, India

    Snehasish Mishra, Biplab Sarkar, Arttatrana Pal, Prangya Paramita Tripathy & Sanjay Kumar Ojha

  3. Ornamental Fish Breeding and Culture Unit, Fish Production Division, CIFA (ICAR), Bhubaneswar, 751 012, Odisha, India

    Saroj Kumar Swain

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  1. Partha Bandyopadhyay
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Correspondence to Snehasish Mishra.

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Partha Bandyopadhyay and Snehasish Mishra: Co-first authors.

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Bandyopadhyay, P., Mishra, S., Sarkar, B. et al. Dietary Saccharomyces cerevisiae Boosts Growth and Immunity of IMC Labeo rohita (Ham.) Juveniles. Indian J Microbiol 55, 81–87 (2015). https://doi.org/10.1007/s12088-014-0500-x

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