Skip to main content
SpringerLink
Log in

Cross-Species Induction and Enhancement of Antimicrobial Properties in Response to Gamma Irradiation in Exiguobacterium sp. HKG 126

  • Original Article
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

A gram positive, extreme haloalkaliphilic, radioresistant bacterium was isolated from mangrove region of Kerala (India) which was characterized as Exiguobacterium sp. HKG-126 using morphological, physiological, biochemical and molecular characterization. Present investigation was undertaken to examine Exiguobacterium sp. as a potential source of broad-spectrum antimicrobial activity and enhancement in this activity was observed due to cross-species/cross-genera induction and also in response to high dose of gamma (γ) irradiation. Individual studies on the antimicrobial activity of all the co-cultivated bacterial strains before and after mixed culture fermentation, showed excellent enhancement in antimicrobial activity of Exiguobacterium sp. against a variety of clinical pathogens. To the best of our knowledge, this is the first report showing existence of an extremely high radioresistant strain of (up to 15 kGy) Exiguobacterium sp.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Demain AL (1998) Induction of microbial secondary metabolism. Int Microbiol 1:259–264

    PubMed  CAS  Google Scholar 

  2. Mearns SA, Bregu M, Boyd KG, Burgess JG (1998) Cross-species induction and enhancement of antimicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria. Lett Appl Microbiol 27:142–146

    Article  Google Scholar 

  3. Slattery M, Rajbhandari I, Wesson K (2001) Competition mediated antibiotic induction in the marine bacterium Streptomyces tenjimariensis. Microb Ecol 41:90–96

    PubMed  CAS  Google Scholar 

  4. Oh DC, Kauffman CA, Jensen PR, Fenical W (2007) Induced production of emericellamides A and B from the marine-derived fungus Emericella sp. in competing co-culture. J Nat Prod 70:515–520

    Article  PubMed  CAS  Google Scholar 

  5. Pettit RK (2009) Mixed fermentation for natural product drug discovery. Appl Microbiol Biotechnol 83:19–25

    Article  PubMed  CAS  Google Scholar 

  6. Waters CM, Bassler BL (2005) Quorum sensing: cell-to-cell communication in bacteria. Annu Rev Cell Dev Biol 21:319–346

    Article  PubMed  CAS  Google Scholar 

  7. Dusane DH, Matkar P, Venugopalan VP, Kumar AR, Zinjarde SS (2011) Cross-species induction of antimicrobial compounds, biosurfactants and quorum-sensing inhibitors in tropical marine epibiotic bacteria by pathogens and biofouling microorganisms. Curr Microbiol 62:974–980

    Article  PubMed  CAS  Google Scholar 

  8. Donadio S, Monciardini P, Alduina R, Mazza P, Chiocchini C, Cavaletti L, Sosio M, Puglia AM (2002) Microbial technologies for the discovery of novel bioactive metabolites. J Biotechnol 99:187–198

    Article  PubMed  CAS  Google Scholar 

  9. Knight V, Sanglier JJ, DiTullio D, Braccili S, Bonner P, Waters J, Hughes D, Zhang L (2003) Diversifying microbial natural products for drug discovery. Appl Microbiol Biotechnol 62:446–458

    Article  PubMed  CAS  Google Scholar 

  10. Genilloud O, Gonzalez I, Salazar O, Martin J, Tormo JR, Vicente F (2011) Current approaches to exploit actinomycetes as a source of novel natural products. J Ind Microbiol Biotechnol 38:375–389

    Article  PubMed  CAS  Google Scholar 

  11. Rakesh OD, Pathak R, Dhaker AS, Arora R, Kumar R, Rajaram R, Gautam HK (2011) Isolation, characterization and bioactivity of deep sea bacteria with special reference to induction of antibacterial and antioxidant metabolites following gamma irradiation. Can J Pure Appl Sci 5:1363–1370

    Google Scholar 

  12. Bergey DH, Holt JG, Kreig NR, Sneath PHA (1994) Bergey’s manual of determinative bacteriology, vol 2, 9th edn. Williams and Wilkins, Baltimore

    Google Scholar 

  13. Gupta AK, Pathak R, Singh B, Gautam H, Kumar R, Kumar R, Arora R, Gautam HK (2011) Proteomic analysis of global changes in protein expression during exposure of gamma radiation in Bacillus sp. HKG-112 isolated from saline soil. J Microbiol Biotechnol 21:574–581

    PubMed  CAS  Google Scholar 

  14. Beaume N, Pathak R, Yadav VK, Kota S, Misra HS, Gautam HK, Chowdhury S (2013) Genome-wide study predicts promoter-G4 DNA motifs regulate selective functions in bacteria: radioresistance of D. radiodurans involves G4 DNA-mediated regulation. Nucleic Acids Res 41(1):76–89

    Article  PubMed  CAS  Google Scholar 

  15. Pathak R, Singh R, Singh A, Gautam H, Dhaker AS, Kumar R, Arora R, Gautam HK (2011) Assessment of antibacterial and free radical scavenging activity in psychrophilic Arthrobacter sp. Pharmacologyonline 1:344–355

    Google Scholar 

  16. Mahato M, Arora V, Pathak R, Gautam HK, Sharma AK (2012) Fabrication of nanostructures through molecular self-assembly of small amphiphilic glyco-dehydropeptides. Mol Biosyst 8:1742–1749

    Article  PubMed  CAS  Google Scholar 

  17. Yang Q, Han Y, Tinh NTN, Hien NT, Bossier P (2012) Detection of quorum sensing signal molecules in edwardsiella ictaluri Ei-151. Indian J Microbiol 52(4):581–586

    Article  CAS  Google Scholar 

  18. Kalia VC, Raju SC, Purohit HJ (2011) Genomic analysis reveals versatile organisms for quorum quenching enzymes: acyl-homoserine lactone-acylase and lactonase. Open Microbiol J 5:1–13

    Article  PubMed  CAS  Google Scholar 

  19. Kalia VC, Purohit HJ (2011) Quenching the quorum sensing system: potential antibacterial drug targets. Crit Rev Microbiol 37(2):121–140

    Article  PubMed  CAS  Google Scholar 

  20. Huma N, Shankar P, Kushwah J, Bhushan A, Joshi J, Mukherjee T, Raju S, Purohit HJ, Kalia VC (2011) Diversity and polymorphism in AHL-lactonase gene (aiiA) of Bacillus. J Microbiol Biotechnol 21(10):1001–1011

    Article  PubMed  CAS  Google Scholar 

  21. Kalia VC (2013) Quorum sensing inhibitors: an overview. Biotechnol Adv. doi:10.1016/j.biotechadv.2012.10.004

  22. Maiden MC (1998) Horizontal genetic exchange, evolution, and spread of antibiotic resistance in bacteria. Clin Infect Dis 27:S12–S20

    Article  PubMed  CAS  Google Scholar 

  23. Udwary DW, Zeigler L, Asolkar RN, Singan V, Lapidus A, Fenical W, Jensen PR, Moore BS (2007) Genome sequencing reveals complex secondary metabolome in the marine actinomycete Salinispora tropica. Proc Natl Acad Sci USA 104:10376–10381

    Article  PubMed  CAS  Google Scholar 

  24. Lal S, Cheema S, Kalia VC (2008) Phylogeny vs genome reshuffling: horizontal gene transfer. Indian J Microbiol 48:228–242

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to Dr. Rajesh Gokhle, director of CSIR-IGIB for providing infrastructural facilities and financial assistance (HKG 61). R. Pathak would also like to acknowledge University Grants Commission (UGC), India, for providing Senior Research Fellowship.

Author information

Authors and Affiliations

  1. Comparative Genomics Unit, CSIR-Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi, 110007, India

    Rajiv Pathak & Hemant K. Gautam

  2. Division of Radiation Biotechnology, Institute of Nuclear Medicine and Allied Sciences (INMAS), DRDO, Timarpur, Delhi, 110007, India

    Raj Kumar

Authors
  1. Rajiv Pathak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Raj Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hemant K. Gautam
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hemant K. Gautam.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pathak, R., Kumar, R. & Gautam, H.K. Cross-Species Induction and Enhancement of Antimicrobial Properties in Response to Gamma Irradiation in Exiguobacterium sp. HKG 126. Indian J Microbiol 53, 130–136 (2013). https://doi.org/10.1007/s12088-013-0369-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-013-0369-0

Keywords

Search

Navigation