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Detection of Quorum Sensing Signal Molecules in Edwardsiella ictaluri Ei-151

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Abstract

Edwardsiella ictaluri is a Gram-negative pathogenic bacterium in the family Enterobacteriaceae that causes enteric septicemia of catfish, which has become a significant problem in the aquaculture of striped catfish (Pangasianodon hypophthalmus) in Vietnam. In this study, a bacterium designated as Ei-151 was isolated from diseased striped catfish and proved to be virulent. Based on 16S rDNA sequencing and phenotypic tests, the pathogenic bacterium was identified as Edw. ictaluri. The presence of quorum sensing signal molecules in Edw. ictaluri Ei-151 was detected with different biosensor strains. The results showed that Ei-151 produced at least three kinds of acylated homoserine lactone (AHL) signal molecules as detected with the biosensor Agrobacterium tumefaciens KYC55, and the AHLs fingerprint was similar to that of Edw. tarda. During its entire growth, the levels of AHLs and autoinducer-2 produced by Ei-151 peaked at the stationary phase (OD600 1.8), which suggested that both of them may function at the stationary phase. No Cholerae autoinducer-1-like activity (including Edw. ictaluri LMG7860T) was detected.

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References

  1. Panangala VS, Shoemaker CA, Klesius PH, Mitra A, Russo R (2009) Cross-protection elicited in channel catfish (Ictalurus punctatus Rafinesque) immunized with a low dose of virulent Edwardsiella ictaluri strains. Aquac Res 40:915–926

    Article  Google Scholar 

  2. USDA (2003) Part II: reference of foodsize catfish health and production practices in the United States, USDA. N.A.H.M.S, Fort Collins

    Google Scholar 

  3. Fuqua WC, Winans SC, Greenberg EP (1994) Quorum sensing in bacteria: the LuxR–LuxI family of cell density-responsive transcriptional regulators. J Bacteriol 176(2):269–275

    PubMed  CAS  Google Scholar 

  4. Irie Y, Parsek MR (2008) Quorum sensing and microbial biofilms. Curr Top Microbiol Immunol 322:67–84

    Article  PubMed  CAS  Google Scholar 

  5. Higgins DA, Pomianek ME, Kraml CM, Taylor RK, Semmelhack MF, Bassler BL (2007) The major Vibrio cholerae autoinducer and its role in virulence factor production. Nature 450:883–886

    Article  PubMed  CAS  Google Scholar 

  6. Defoirdt T, Verstraete W, Bossier P (2008) Luminescence, virulence and quorum sensing signal production by pathogenic Vibrio campbellii and Vibrio harveyi isolates. J Appl Microbiol 104:1480–1487

    Article  PubMed  CAS  Google Scholar 

  7. Kleerebezem M (2004) Quorum sensing control of lantibiotic production; nisin and subtilin autoregulate their own biosynthesis. Peptides 25:1405–1414

    Article  PubMed  CAS  Google Scholar 

  8. Lazazzera BA (2000) Quorum sensing and starvation: signals for entry into stationary phase. Curr Opin Microbiol 3:177–182

    Article  PubMed  CAS  Google Scholar 

  9. Waters CM, Bassler BL (2005) Quorum sensing: cell-to-cell communication in bacteria. Annu Rev Cell Dev Biol 21:319–346

    Article  PubMed  CAS  Google Scholar 

  10. Chen X, Schauder S, Potier N, Van Dorssealaer A, Pelczer I, Bassler BL, Hughson FM (2002) Structural identification of a bacterial quorum-sensing signal containing boron. Nature 415:545–549

    Article  PubMed  CAS  Google Scholar 

  11. Han Y, Li X, Qi ZZ, Zhang XH, Bossier P (2010) Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4. J Appl Microbiol 108(1):139–147

    Article  PubMed  CAS  Google Scholar 

  12. Zhang M, Sun K, Sun L (2008) Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain. Microbiology 154:2060–2069

    Article  PubMed  CAS  Google Scholar 

  13. Tempe J, Petit A, Holsters M, Mon Atagu MV, Schell J (1977) Thermosensitive step associated with transfer of the Ti Plasmid during conjugation: possible relation to transformation in crown gall. Proc Natl Acad Sci USA 74:2848–2849

    Article  PubMed  CAS  Google Scholar 

  14. Greenberg EP, Hastings JW, Ulitzur S (1979) Induction of luciferase synthesis in Beneckea harveyi by other marine bacteria. Arch Microbiol 120:87–91

    Article  CAS  Google Scholar 

  15. Reed LJ, Muench S (1938) A simple method of estimating fifty percent and point. Am J Hyg 27:493–497

    Google Scholar 

  16. Lan J, Zhang XH, Wang Y, Chen J, Han Y (2008) Isolation of an unusual strain of Edwardsiella tarda from turbot and establish a PCR detection technique with the gyrB gene. J Appl Microbiol 105:644–651

    Article  PubMed  CAS  Google Scholar 

  17. Garrity GM (2005) Bergey’s manual of systematic bacteriology, vol 2, 2nd edn. Springer, New York

    Google Scholar 

  18. Zhu J, Chai Y, Zhong Z, Li S, Winans SC (2003) Agrobacterium bioassay strain for ultrasensitive detection of N-acylhomoserine lactone-type quorum-sensing molecules: detection of autoinducers in Mesorhizobium huakuii. Appl Environ Microbiol 69:6949–6953

    Article  PubMed  CAS  Google Scholar 

  19. Vilchez R, Lemme A, Thiel V, Schulz S, Sztajer H, Wagner DI (2007) Analysing traces of autoinducer-2 requires standardization of the Vibrio harveyi bioassay. Anal Bioanal Chem 387:489–496

    Article  PubMed  CAS  Google Scholar 

  20. Panangala VS, Shoemaker CA, McNulty ST, Arias CR, Klesius PH (2006) Intra- and interspecific phenotypic characteristics of fish-pathogenic Edwardsiella ictaluri and E. tarda. Aquac Res 37:49–60

    Article  CAS  Google Scholar 

  21. Pesci EC, Iglewski BH (1997) The chain of command in Pseudomonas quorum sensing. Trends Microbiol 5:132–135

    Article  PubMed  CAS  Google Scholar 

  22. You Z, Fukushima J, Tanaka K, Kawamoto S, Okuda K (1998) Induction of entry into the stationary growth phase in Pseudomonas by N-acylhomoserine lactone. FEMS Microbiol Lett 164:99–106

    Article  PubMed  CAS  Google Scholar 

  23. Kalia VC, Raju SC, Purohit HJ (2011) Genomic analysis reveals versatile organisms for quorum quenching enzymes: acyl-homoserine lactone- acylase and -lactonase. Open Microbiol J 5:1–13

    Article  PubMed  CAS  Google Scholar 

  24. Huma N, Shankar P, Kushwah J, Bhushan A, Joshi J, Mukherjee T, Raju SC, Purohit HJ, Kalia VC (2011) Diversity and polymorphism in AHL-lactonase gene (aiiA) of Bacillus. J Microbiol Biotechnol 21:1001–1011

    Article  PubMed  CAS  Google Scholar 

  25. Kalia VC, Purohit HJ (2011) Quenching the quorum sensing system: potential antibacterial drug targets. Crit Rev Microbiol 37:121–140

    Article  PubMed  CAS  Google Scholar 

  26. Vendeville A, Winzer K, Heurlier K, Tang CM, Hardie KR (2005) Making ‘sense’ of metabolism: autoinducer-2, LuxS and pathogenic bacteria. Nat Rev Microbiol 3:383–396

    Article  PubMed  CAS  Google Scholar 

  27. Lombardía E, Rovetto AJ, Arabolaza AL, Grau RR (2006) A LuxS-dependent cell-to-cell language regulates social behavior and development in Bacillus subtilis. J Bacteriol 188:4442–4452

    Article  PubMed  Google Scholar 

  28. Henke JM, Bassler BL (2004) Three parallel quorumsensing systems regulate gene expression in Vibrio harveyi. J Bacteriol 186:6902–6914

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The work was supported by grants from the National Natural Science Foundation of China (No. 31072241).

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China

    Qian Yang & Yin Han

  2. Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium

    Qian Yang & Peter Bossier

  3. Research Institute for Aquaculture No. 2, Hochiminh City, Vietnam

    Nguyen Thi Ngoc Tinh & Nguyen Thi Hien

Authors
  1. Qian Yang
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  2. Yin Han
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  3. Nguyen Thi Ngoc Tinh
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  4. Nguyen Thi Hien
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  5. Peter Bossier
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Corresponding author

Correspondence to Yin Han.

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Yang, Q., Han, Y., Tinh, N.T.N. et al. Detection of Quorum Sensing Signal Molecules in Edwardsiella ictaluri Ei-151. Indian J Microbiol 52, 581–586 (2012). https://doi.org/10.1007/s12088-012-0312-9

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  • DOI: https://doi.org/10.1007/s12088-012-0312-9

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