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Hepatocyte-like cells derived from induced pluripotent stem cells

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Abstract

The discovery that coordinated expression of a limited number of genes can reprogram differentiated somatic cells to induced pluripotent stem cells (iPSC) has opened novel possibilities for developing cell-based models of diseases and regenerative medicine utilizing cell reprogramming or cell transplantation. Directed differentiation of iPSCs can potentially generate differentiated cells belonging to any germ layer, including cells with hepatocyte-like morphology and function. Such cells, termed iHeps, can be derived by sequential cell signaling using available information on embryological development or by forced expression of hepatocyte-enriched transcription factors. In addition to the translational aspects of iHeps, the experimental findings have provided insights into the mechanisms of cell plasticity that permit one cell type to transition to another. However, iHeps generated by current methods do not fully exhibit all characteristics of mature hepatocytes, highlighting the need for additional research in this area. Here we summarize the current approaches and achievements in this field and discuss some existing hurdles and emerging approaches for improving iPSC differentiation, as well as maintaining such cells in culture for increasing their utility in disease modeling and drug development.

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  1. Division of Gastroenterology and Hepatology, Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, 10461, USA

    Namita Roy-Chowdhury, Xia Wang & Jayanta Roy-Chowdhury

  2. Marion Bessin Liver Research Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, 10461, USA

    Namita Roy-Chowdhury, Xia Wang, Chandan Guha & Jayanta Roy-Chowdhury

  3. Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, 10461, USA

    Namita Roy-Chowdhury & Jayanta Roy-Chowdhury

  4. Department of Radiation Oncology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, 10461, USA

    Chandan Guha

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This work was funded in part by the following Grants: 1PO1 DK 096990-01 (to JRC, PD: D. Perlmutter); RO1 DK092469 (to NRC), P30 DK041296-28 (to JRC, A. PD: Wolkoff) R01 DK100490-01A1 (to JRC, PI: DA Shafritz); New York Stem Cell Foundation CO26440 (to JRC); R01 DK064670, R33 CA121051 (to CG).

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Namita Roy-Chowdhury, Xia Wang, Chandan Guha and Jayanta Roy-Chowdhury declare that they have no conflict of interest.

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This article does not contain any studies with human participants or any studies with animals performed by any of the authors.

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Roy-Chowdhury, N., Wang, X., Guha, C. et al. Hepatocyte-like cells derived from induced pluripotent stem cells. Hepatol Int 11, 54–69 (2017). https://doi.org/10.1007/s12072-016-9757-y

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  • DOI: https://doi.org/10.1007/s12072-016-9757-y

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