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Complement component 3: characterization and association with mastitis resistance in Egyptian water buffalo and cattle

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Abstract

Mastitis is an infectious disease of the mammary gland that leads to reduced milk production and change in milk composition. Complement component C3 plays a major role as a central molecule of the complement cascade involving in killing of microorganisms, either directly or in cooperation with phagocytic cells. C3 cDNA were isolated, from Egyptian buffalo and cattle, sequenced and characterized. The C3 cDNA sequences of buffalo and cattle consist of 5025 and 5019 bp, respectively. Buffalo and cattle C3 cDNAs share 99% of sequence identity with each other. The 4986 bp open reading frame in buffalo encodes a putative protein of 1661 amino acids—as in cattle—and includes all the functional domains. Further, analysis of the C3 cDNA sequences detected six novel single-nucleotide polymorphisms (SNPs) in buffalo and three novel SNPs in cattle. The association analysis of the detected SNPs with milk somatic cell score as an indicator of mastitis revealed that the most significant association in buffalo was found in the C >A substitution (ss: 1752816097) in exon 27, whereas in cattle it was in the C >T substitution (ss: 1752816085) in exon 12. Our findings provide preliminary information about the contribution of C3 polymorphisms to mastitis resistance in buffalo and cattle.

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Acknowledgements

This study was funded by the Science and Technology Development Fund (STDF) in Egypt (grant no. 2047).

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Authors and Affiliations

  1. Department of Cell Biology, National Research Centre, 12622, Giza, Egypt

    NERMIN EL-HALAWANY, ABD-EL-MONSIF A. SHAWKY & AHMED F. M. AL-TOHAMY

  2. Avisina Group, Benha, Qalubia, 13776, Egypt

    LAMEES HEGAZY

  3. Faculty of Agriculture, Department of Animal Production, Cairo University, 12613, Giza, Egypt

    HAMDY ABDEL-SHAFY

  4. Department of Buffalo Research, Animal Production Research Institute, Doki, 12126, Giza, Egypt

    MAGDY A. ABDEL-LATIF

  5. Department of Animal Reproduction and A.I. National Research Centre, 12622, Giza, Egypt

    YASSER A. GHAZI

  6. Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, 53115, Germany

    CHRISTIANE NEUHOFF, DESSIE SALILEW-WONDIM & KARL SCHELLANDER

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  1. NERMIN EL-HALAWANY
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Correspondence to NERMIN EL-HALAWANY.

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Corresponding editor: Silvia Garagna

[El-Halawany N., Abd-El-Morif S. A., Al-Tohamy A. F. M., Hegazy L., Abdel-Shafy H., Abdel-Latif M. A., Ghazi Y. A., Neuhoff C., Salilew-Wondim D. and Schellander K. 2017 Complement component 3: characterization and association with mastitis resistance in Egyptian water buffalo and cattle. J. Genet. 96, xx–xx]

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EL-HALAWANY, N., SHAWKY, AEM.A., M. AL-TOHAMY, A.F. et al. Complement component 3: characterization and association with mastitis resistance in Egyptian water buffalo and cattle. J Genet 96, 65–73 (2017). https://doi.org/10.1007/s12041-017-0740-8

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