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Sequence variants of the LCORL gene and its association with growth and carcass traits in Qinchuan cattle in China

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Abstract

Molecular marker-assisted selection is a better way to satisfy the growing customer requirement with the development of beef cattle growth and breeding research. For now, quantitative trait locus (QTL) for cattle growth and carcass traits, just like body height, body length and carcass weight have been detected on bovine chromosome 6. In this study, ligand-dependent nuclear receptor corepressor-like (LCORL) was selected as the potential positional candidate gene located in chromosome 6 which is closely connected with the bovine growth and carcass traits. A total of 450 Qinchuan beef cattle were used to detect mutations in exon and its neighbouring region, and the promoter region of the bovine LCORL gene. The methods for SNPs detection were polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and created restriction site PCR (CRS-PCR), and the results of this study show that there were two variations in intron regions, the other four variations were located in the promoter region. Linkage disequilibrium analysis and haplotype analysis indicated that L78-Q4 had strong linkage disequilibrium, A T G C G C (16.2%) and G C G C A T (16.7%) had higher haplotype frequencies, G C A C A C (0.8%) and G T A C A T (0.7%) had lower haplotype frequencies. Correlation analysis indicated that SNP g. INT + 52098A >G was significantly associated with slaughter weight and carcass weight. Based on the research, we selected LCORL as the candidate gene that can contribute to improved marker-assisted selection for the meat performance of Qinchuan beef cattle.

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Acknowledgements

This research was supported by the National Eleventh ‘Five-Year’ National Science and Technology Key Project (no. 2008AA101010), National Twelfth ‘Five-Year’ National Science and Technology Key Project (no. 2011AA100307), ‘13115’ Sci-Tech Innovation Programme of Shaanxi province (no. 2008ZDKG-11) and Sci-Tech Integrated Innovation Engineering Projects of Shaanxi province (no. S2015TLNY0017).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, No. 22, Xinong Road, Yangling, Shaanxi, 712100, People’s Republic of China

    Y. J. HAN, Y. CHEN, Y. LIU & X. L. LIU

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  1. Y. J. HAN
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  2. Y. CHEN
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  3. Y. LIU
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Correspondence to X. L. LIU.

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Corresponding editor: Silvia Garagna

[Han Y. J., Chen Y., Liu Y. and Liu X. L. 2017 Sequence variants of the LCORL gene and its association with growth and carcass traits in Qinchuan cattle in China. J. Genet. 96, xx–xx]

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HAN, Y.J., CHEN, Y., LIU, Y. et al. Sequence variants of the LCORL gene and its association with growth and carcass traits in Qinchuan cattle in China. J Genet 96, 9–17 (2017). https://doi.org/10.1007/s12041-016-0732-0

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  • DOI: https://doi.org/10.1007/s12041-016-0732-0

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