Abstract
To investigate allelic variation of Myb10-1 genes in Chinese wheat and to examine its association with germination level in wheat, a total of 582 Chinese bread wheat cultivars and 110 Aegilops tauschii accessions were used to identify allelic variations of three Myb10-1 genes. Identification results indicated that there is a novel Tamyb10-B1 allele, designated Tamyb10-B1c, in the five Chinese landraces. The Tamyb10-B1c possibly has a large deletion including Tamyb10-B1 gene. There are three novel Tamyb10-D1 alleles (Aetmyb10-D1c, Aetmyb10-D1d and Aetmyb10-D1e) that were discovered in Aegilops tauschii.. Of them, Aetmyb10-D1c allele possessed a 104-bp deletion and this resulted in a frame shift in the open reading frame of the Aetmyb10-D1 gene. AETMYB10-D1d and AETMYB10-D1e proteins possessed three and two different amino acids when compared with TAMYB10-D1b protein, respectively. Association of Tamyb10-1 allelic variation with grain germination level indicated that all five allelic combinations with red grains showed a significantly higher GP (germination percentage) and GI (germination index) values than those of white-grained Tamyb10-A1a/Tamyb10-B1a/Tamyb10-D1a genotype after storing it for one year. Moreover, the Tamyb10-A1b/Tamyb10-B1c/Tamyb10-D1b genotype possesses the significantly highest GP and GI among the six different Tamyb10-1 combinations. This study could provide useful information for wheat breeding programme in terms of grain colour and germination level.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chen F., He Z. H., Xia X. C., Xia L. Q., Zhang X. Y., Lillemo M. et al. 2006 Molecular and biochemical characterization of puroindoline a and b alleles in Chinese landraces and historical cultivars. Theor. Appl. Genet. 112, 400–409.
Chen F., Xu H. X., Zhang F. Y., Xia X. C., He Z. H., Wang D. W. et al. 2011 Physical mapping of puroindoline b-2 genes and molecular characterization of a novel variant in durum wheat (Triticum turgidum L.) Mol. Breed. 28, 153–161.
Dvorak J., Luo M. C., Yang Z. L. and Zlmng H. B. 1998 The structure of the Aegilops tauschii gene pool and the evolution of hexaploid wheat. Theor. Appl. Genet. 97, 657–670.
Flintham J. E. 2000 Different genetic components control coatimposed and embryo-imposed dormancy in wheat. Seed Sci. Res. 10, 43–50.
Groos C., Gay G., Perretant M. R., Gervais L., Bernard M., Dedryver F. et al. 2002 Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white ×red grain bread-wheat cross. Theor. Appl. Genet. 104, 39–47.
Grotewold E., Drummond B. J., Bowen B. and Peterson T. 1994 Themyb homologous P gene controls phlobaphene pigmentation in maize floral organs by directly activating a flavonoid biosynthetic gene subset. Cell 76, 543–553.
Himi E. and Noda K. 2005 Red grain colour gene (R) of wheat is a myb type transcription factor. Euphytica 143, 239–242.
Himi E., Maekawa M., Miura H. and Noda K. 2011 Development of PCR markers for Tamyb10 related to R-1, red grain color gene in wheat. Theor. Appl. Genet. 122, 1561–1576.
Himi E., Mares D. J., Yanagisawa A. and Noda K. 2002 Effect of grain color gene (R) on grain dormancy and sensitivity of the embryo to abscisic acid (ABA) in wheat. J. Exp. Bot. 53, 1569–1574.
Himi E., Nisar A. and Noda K. 2005 Colour genes (R and Rc) for grain and coleoptile upregulate flavonoid biosynthesis genes in wheat. Genome 48, 747–754.
Jia J., Zhao S., Kong X., Li Y., Zhao G., He W. et al. 2013 Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation. Nature 496, 91–95.
Kottearachchi N. S., Takao S., Kato K. and Miura H. 2008 Mapping of a QTL in chromosome 3B for grain dormancy in white-grained wheat population. J. Food Agric. 1, 1–10.
Lepiniec L., Debeaujon I., Routaboul J. M., Baudry A., Pourcel L, Nesi N. et al. 2006 Genetics and biochemistry of seed flavonoids. Annu. Rev. Plant. Biol. 57, 405–430.
Li J., Wei H. T., Hu X. R., Lu B. R. and Yang W. Y. 2010 Locus R-D1 conferring red-grain-color in synthetic derivative wheatChuanmai 42 mapped with SSR markers. Mol. Plant. Breed. 3, 1–6.
Lohwasser U., Rehman-Arif M. A. and Börner A. 2013 Discovery of loci determining pre-harvest sprouting and dormancy in wheat and barley applying segregation and association mapping. Biol. Plant. 57, 663–674.
Miyamoto T. and Everson E. H. 1958 Biochemical and physiological studies of wheat seed pigmentation. Agron. J. 50, 733–734.
Nesi N., Jond C., Debeaujon I., Caboche M. and Lepiniec L. 2001 The Arabidopsis TT2 gene encodes an R2R3 MYB domain protein that acts as a key determinant for proanthocyanidin accumulation in developing seed. Plant Cell 13, 2099–2114.
Rehman-Arif M. A., Neumann K., Kobiljski B., Nagel M., Lohwasser U. and Börner A. 2012 An association mapping study of dormancy and pre-harvest sprouting in wheat. Euphytica 188, 409–417.
Van Slageren M. W. 1994 Wild wheats: a monograph of Aegilops L. and Amblyopyrum (Jaub. &Spach) Eig (Poaceae), pp. 94–97. Wagningen Agricultural University, Wageningen, the Netherlands.
Walker-Simmons M. K. 1988 Enhancement of ABA responsiveness in wheat embryos at higher temperature. Plant Cell Environ. 11, 769–775.
Warner R. L., Kudrna D. A., Spaeth S. C. and Jones S. S. 2000 Dormancy in white-grain mutants of Chinese Spring wheat (Triticum aestivum L.) Seed Sci. Res. 10, 51–60.
Wei H. T., Li J., Peng Z. S., Lu B. R., Zhao Z. J. and Yang W. Y. 2008 Relationships of Aegilops tauschii revealed by DNA fingerprints: The evidence for agriculture exchange between China and the West. Progress Nat. Sci. 18, 1525–1531.
Winkel-Shirley B. 2001 Flavonoid biosynthesis: A colourful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol. 126, 485–493.
Xia L. Q., Chen F., He Z. H., Chen X. M. and Morris C. F. 2005 Occurrence of puroindoline alleles in Chinese winter wheats. Cereal Chem. 82, 38–43.
Yen C., Yang J. L., Liu X. D. and Li L. R. 1983 The distribution of Aegilops tauschii Cosson in China with reference of the origin of the Chinese common wheat. In Proceedings of the 6th International Wheat Genet Syrup, pp. 55–58. Kyoto, Japan.
Acknowledgements
This project was funded by the 973 projects (2014CB160303 and 2014CB138105), Programmes for New Century Excellent Talents in University (NCET-13-0776) and Henan University Science and Technological Innovation Team (14IRTSTHN010) of China.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Zhong Dong Dong, Jie Chen and Ting Li contributed equally to this work.
[Dong Z. D., Chen J., Li T., Chen F. and Cui D. Q. 2015 Molecular survey of Tamyb10-1 genes and their association with grain colour and germinability in Chinese wheat and Aegilops tauschii. J. Genet. 94, xx–xx]
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
DONG DONG, Z., CHEN, J., LI, T. et al. Molecular survey of Tamyb10-1 genes and their association with grain colour and germinability in Chinese wheat and Aegilops tauschii . J Genet 94, 453–459 (2015). https://doi.org/10.1007/s12041-015-0559-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12041-015-0559-0