Journal of Genetics

, Volume 92, Issue 3, pp 545–557

Assessment of genetic diversity in Indian rice germplasm (Oryza sativa L.): use of random versus trait-linked microsatellite markers

  • SHEEL YADAV
  • ASHUTOSH SINGH
  • M. R. SINGH
  • NITIKA GOEL
  • K. K. VINOD
  • T. MOHAPATRA
  • A. K. SINGH
Research Article

DOI: 10.1007/s12041-013-0312-5

Cite this article as:
YADAV, S., SINGH, A., SINGH, M.R. et al. J Genet (2013) 92: 545. doi:10.1007/s12041-013-0312-5

Abstract

Assessment of genetic diversity in a crop germplasm is a vital part of plant breeding. DNA markers such as microsatellite or simple sequence repeat markers have been widely used to estimate the genetic diversity in rice. The present study was carried out to decipher the pattern of genetic diversity in terms of both phenotypic and genotypic variability, and to assess the efficiency of random vis-à-vis QTL linked/gene based simple sequence repeat markers in diversity estimation. A set of 88 rice accessions that included landraces, farmer’s varieties and popular Basmati lines were evaluated for agronomic traits and molecular diversity. The random set of SSR markers included 50 diversity panel markers developed under IRRI’s Generation Challenge Programme (GCP) and the trait-linked/gene based markers comprised of 50 SSR markers reportedly linked to yield and related components. For agronomic traits, significant variability was observed, ranging between the maximum for grains/panicle and the minimum for panicle length. The molecular diversity based grouping indicated that varieties from a common centre were genetically similar, with few exceptions. The trait-linked markers gave an average genetic dissimilarity of 0.45 as against that of 0.37 by random markers, along with an average polymorphic information constant value of 0.48 and 0.41 respectively. The correlation between the kinship matrix generated by trait-linked markers and the phenotype based distance matrix (0.29) was higher than that of random markers (0.19). This establishes the robustness of trait-linked markers over random markers in estimating genetic diversity of rice germplasm.

Keywords

germplasm genetic diversity microsatellite markers principal component analysis 

Supplementary material

12041_2013_312_MOESM1_ESM.pdf (514 kb)
(PDF 514 KB)

Copyright information

© Indian Academy of Sciences 2013

Authors and Affiliations

  • SHEEL YADAV
    • 1
    • 5
  • ASHUTOSH SINGH
    • 1
  • M. R. SINGH
    • 2
  • NITIKA GOEL
    • 1
  • K. K. VINOD
    • 3
  • T. MOHAPATRA
    • 4
  • A. K. SINGH
    • 1
  1. 1.Division of GeneticsIndian Agricultural Research InstituteNew DelhiIndia
  2. 2.Post Graduate CollegeGhazipurIndia
  3. 3.Indian Agricultural Research InstituteRice Breeding and Genetics Research CentreAduthuraiIndia
  4. 4.Central Rice Research InstituteCuttackIndia
  5. 5.Division of Genomic ResourcesNational Bureau of Plant Genetic ResourcesNew DelhiIndia

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