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Genetic diversity studies and identification of SSR markers associated with Fusarium wilt (Fusarium udum) resistance in cultivated pigeonpea (Cajanus cajan)

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Abstract

Genetic diversity and identification of simple sequence repeat markers correlated with Fusarium wilt resistance was performed in a set of 36 elite cultivated pigeonpea genotypes differing in levels of resistance to Fusarium wilt. Twenty-four polymorphic sequence repeat markers were screened across these genotypes, and amplified a total of 59 alleles with an average high polymorphic information content value of 0.52. Cluster analysis, done by UPGMA and PCA, grouped the 36 pigeonpea genotypes into two main clusters according to their Fusarium wilt reaction. Based on the Kruskal–Wallis ANOVA and simple regression analysis, six simple sequence repeat markers were found to be significantly associated with Fusarium wilt resistance. The phenotypic variation explained by these markers ranged from 23.7 to 56.4%. The present study helps in finding out feasibility of prescreened SSR markers to be used in genetic diversity analysis and their potential association with disease resistance.

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References

  • Bohra A., Saxena R. K., Gnanesh B. N., Saxena K. B., Byregowda M., Rathore A. et al. 2012 An intra-specific consensus genetic map of pigeonpea [Cajanus cajan (L.) Millspaugh] derived from six mapping populations. Theor. Appl. Genet. 125, 1325–1338.

    Article  PubMed  Google Scholar 

  • Burns M. J., Edwards K. J., Newbury H. J., Ford-Lloyd B. R. and Baggot C. D. 2001 Development of simple sequence repeat (SSR) markers for the assessment of gene flow and genetic diversity in pigeonpea (Cajanus cajan). Mol. Ecol. Notes 1, 283–285.

    Article  CAS  Google Scholar 

  • Dutta S., Kumawat G., Singh B. P., Gupta D. K., Singh S., Dogra V. et al. 2011 Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L.) Millspaugh]. BMC Plant Biol. 11, 17.

    Article  PubMed  CAS  Google Scholar 

  • Gnanesh B. N., Bohra A., Sharma M., Byregowda M., Pande S., Wesley V. et al. 2011 Genetic mapping and quantitative trait locus analysis of resistance to sterility mosaic disease in pigeonpea [Cajanus cajan (L.) Millsp.]. Field Crops Res. 123, 56–61.

    Article  Google Scholar 

  • Gwata E. T., Silim S. N. and Mgonja M. 2006 Impact of a new source of resistance to Fusarium wilt in pigeonpea. J. Phytopathol. 154, 62–64.

    Article  Google Scholar 

  • Hammer Ø., Harper D. A. T. and Ryan P. D. 2001 PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontol. Electron. 4, 9.

    Google Scholar 

  • Jaccard P. 1908 Nouvelle srecherches sur la distribution florale. Bull. Soc. Vaud. Sci. Nat. 44, 223–270.

    Google Scholar 

  • Kotresh H., Fakrudin B., Punnuri S. M., Rajkumar B. K., Thudi M., Paramesh H. et al. 2006 Identification of two RAPD markers genetically linked to a recessive allele of a Fusarium wilt resistance gene in pigeonpea (Cajanus cajan L. Millsp.). Euphytica 149, 113–120.

    Article  CAS  Google Scholar 

  • Kumar S., Gupta S., Chandra S. and Singh B. B. 2004 How wide is the genetic base of pulse crops. In Pulses in new perspective (ed. M. Ali, B. B. Singh, S. Kumar and Vishwadhar), pp. 211–221. IIPR, Kanpur, India.

  • Mace E. S., Phong D. T., Upadhyaya H. D., Chandra S. and Crouch J. H. 2006 SSR analysis of cultivated groundnut (Arachis hypogaea L.) germplasm resistant to rust and late leaf spot diseases. Euphytica 152, 317–330.

    Article  Google Scholar 

  • Messmer M. M., Melchinger A. E., Herrmann R. G. and Boppenmaier J. 1993 Relationships among early European maize inbreds: II. Comparison of pedigree and RFLP data. Crop Sci. 33, 944–950.

    Article  Google Scholar 

  • Mir R. R., Saxena R. K., Saxena K. B., Upadhyaya H. D., Kilian A., Cook D. R. and Varshney R. K. 2012 Whole-genome scanning for mapping determinacy in Pigeonpea (Cajanus spp.). Plant Breed. (doi:10.1111/j.1439-0523.2012.02009.x).

    Google Scholar 

  • Mondal S. and Badigannavar A. M. 2010 Molecular diversity and association of SSR markers to rust and late leaf spot resistance in cultivated groundnut (Arachis hypogaea L.). Plant Breed. 129, 68–71.

    Article  CAS  Google Scholar 

  • Nadimpalli R. G., Jarret R. L., Phatak S. C. and Kochart G. 1994 Phylogenetic relationships of the pigeonpea (Cajanus cajan) based on nuclear restriction fragment length polymorphism. Genome 36, 216–223.

    Article  Google Scholar 

  • Nene Y. L. and Kannnaiyan J. 1982 Screening pigeonpea for resistance to Fusarium wilt. Plant Dis. 66, 306–307.

    Article  Google Scholar 

  • Odeny D. A., Jayashree B., Ferguson M., Hoisington D., Cry L. J. and Gebhardt C. 2007 Development, characterization and utilization of microsatellite markers in pigeonpea. Plant Breed. 126, 130–136.

    Article  CAS  Google Scholar 

  • Odeny D. A., Jayashree B., Gebhardt C. and Crouch J. 2009 New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.). BMC Res. Notes 2, 35.

    Article  PubMed  CAS  Google Scholar 

  • Okiror M. A. 2002 Genetics of resistance to Fusarium udum in pigeonpea [Cajanus cajan (L.) Millsp.]. Indian J. Genet. Plant Breed. 62, 218–220.

    Google Scholar 

  • Panguluri S. K., Janaiah K., Govil J. N., Kumar P. A. and Sharma P. C. 2006 AFLP fingerprinting in pigeonpea (Cajanus cajan (L.) Millsp.) and its wild relatives. Genet. Resour. Crop Evol. 53, 523–531.

    Article  Google Scholar 

  • Prasad P., Eswara Reddy N. P., Anandam R. J. and Lakshmikantha R. G. 2003 Isozymes variability among Fusarium udum resistant cultivars of pigeonpea (Cajanus cajan (L.) (Millsp). Acta Physiol. Plant 25, 221–228.

    Article  CAS  Google Scholar 

  • Reddy L. J., Upadhyaya H. D., Gowda C. L. L. and Singh S. 2005 Development of a core collection in pigeonpea [Cajanus cajan (l.) Millspaugh] using geographic and qualitative morphological descriptors. Genet. Resour. Crop Evol. 52, 1049–1056.

    Article  Google Scholar 

  • Rohlf F. J. 1997 NTSYS-pc (numerical taxonomy and multivariate analysis system), version 2.02i, Exeter, Setauket, New York, USA.

  • SAS Institute Inc. 1998 SAS/STAT user’s guide, release 6.03 ed. SAS Institute, Cary, USA.

  • Saxena K. B. and Sharma D. 1990 Pigeonpea: genetics. In: The pigeonpea (ed. Y. L. Nene, S. D. Hall and V. K. Sheila). Wallingford, Oxon, England.

    Google Scholar 

  • Saxena K. B., Sultana R., Mallikarjuna N., Saxena R. K., Kumar R. V., Sawargaonkar S. L. and Varshney R. K. 2010 Male-sterility systems in pigeonpea and their role in enhancing yield. Plant Breed. 129, 125–134.

    Article  Google Scholar 

  • Saxena R. K., Cui X., Thakur V., Walter B., Close T. J. and Varshney R. K. 2011 Single feature polymorphisms (SFPs) for drought tolerance in pigeonpea (Cajanus spp.). Funct. Integr. Genomics 11, 651–657.

    Article  PubMed  CAS  Google Scholar 

  • Singh A. K., Rai V. P., Singh M. N., Chand R., Ramdhari and Singh R. M. 2012 Genetic diversity analysis in Cajanus species using SSR markers presented at the VIth International Conference on Legume Genetics and Genomics, Hyderabad, India.

  • Sivaramakrishnan S., Kannan S. and Reddy L. J. 2002 Diversity in selected wild and cultivated species of pigeonpea using RFLP of mtDNA. Euphytica 125, 21–28.

    Article  CAS  Google Scholar 

  • Sneath P. H. A. and Sokal R. R. 1973 Numerical taxonomy. Freeman, San Francisco, USA.

    Google Scholar 

  • Varshney R. K., Chen W., Li Y., Bharti A. K., Saxena R. K., Schlueter J. A. et al. 2012 Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource poor farmers. Nat. Biotechnol. 30, 83–89.

    Article  CAS  Google Scholar 

  • Varshney R. K., Hoisington D. A. and Tyagi A. K. 2006 Advances in cereal genomics and applications in crop breeding. Trends Biotechnol. 24, 490–499.

    Article  PubMed  CAS  Google Scholar 

  • Wasike S., Okori P. and Rubaihayo P. R. 2005 Genetic variability and relatedness of the Asian and African pigeonpea as revealed by AFLP. Afr. J. Biotechnol. 4, 1228–1233.

    CAS  Google Scholar 

  • Yadav K., Yadav S. K., Yadav A., Pandey V. P. and Dwivedi U. N. 2012 Genetic diversity of pigeonpea (cajanus cajan (l.) Millsp.) cultivars and its wild relatives using randomly amplified polymorphic DNA (RAPD) markers. Am. J. Plant. Sci. 3, 322–330.

    Article  CAS  Google Scholar 

  • Yang S. Y., Pang W., Ash G., Harper J., Carling J., Wenzl P. et al. 2006 Low level of genetic diversity in cultivated pigeonpea compared to its wild relatives is revealed by diversity arrays technology. Theor. Appl. Genet. 113, 585–595.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

This work was done under ICAR funded research project ‘Niche Area of Excellence-Molecular Breeding for Improvement of Major Crops of Eastern Indo-Gangetic Plains’. Financial assistance provided by ICAR, New Delhi is duly acknowledged.

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanas, 221 005, India

    A. K. SINGH, V. P. RAI, R. P. SINGH & M. N. SINGH

  2. Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanas, 221 005, India

    R. CHAND

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  1. A. K. SINGH
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  2. V. P. RAI
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  3. R. CHAND
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  4. R. P. SINGH
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Correspondence to M. N. SINGH.

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Singh A. K., Rai V. P., Chand R., Singh R. P. and Singh M. N. 2013 Genetic diversity studies and identification of SSR markers associated with Fusarium wilt (Fusarium udum) resistance in cultivated pigeonpea (Cajanus cajan). J. Genet. 92, xx–xx

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SINGH, A.K., RAI, V.P., CHAND, R. et al. Genetic diversity studies and identification of SSR markers associated with Fusarium wilt (Fusarium udum) resistance in cultivated pigeonpea (Cajanus cajan). J Genet 92, 273–280 (2013). https://doi.org/10.1007/s12041-013-0266-7

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  • DOI: https://doi.org/10.1007/s12041-013-0266-7

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