Journal of Genetics

, 87:65

Molecular assessment of genetic diversity in mung bean germplasm

  • G. Roopa Lavanya
  • Jyoti Srivastava
  • Shirish A. Ranade
Research Article

DOI: 10.1007/s12041-008-0009-3

Cite this article as:
Lavanya, G.R., Srivastava, J. & Ranade, S.A. J Genet (2008) 87: 65. doi:10.1007/s12041-008-0009-3

Abstract

RAPD profiles were used to identify the extent of diversity among 54 accessions of mung bean that included both improved and local land races. Out of the 40 primers screened, seven primers generated 174 amplification products with an average of 24.85 bands per primer. The RAPD profiles were analysed for Jaccard’s similarity coefficients that was found to be in the range from 0 to 0.48, indicating the presence of wide range of genetic diversity at molecular level. Cluster analysis was carried out based on distances (1-similarity coefficient) using neighbour-joining method in Free Tree package. The dendrogram resolved all the accessions into two major clusters, I (with 11 accessions) and II (with 43 accessions). However, the cluster was further divided into four subclusters (II A with six, II B with nine, II C with 15 and II D with 13 accessions). The distribution of the accessions in different clusters and subclusters appears to be related to their performance in field conditions for 10 morphological traits that were scored. This study indicated that the RAPD profiles provide an easy and simple technique for preliminary genetic diversity assessment of mung bean accessions that may reflect morphological trait differences among them.

Keywords

genetic diversitylocal land racesmung beanfield performanceRAPD

Copyright information

© Indian Academy of Sciences 2008

Authors and Affiliations

  • G. Roopa Lavanya
    • 1
  • Jyoti Srivastava
    • 2
  • Shirish A. Ranade
    • 2
  1. 1.Department of Genetics and Plant BreedingAllahabad Agricultural Institute-Deemed UniversityAllahabadIndia
  2. 2.PMB (Genomics)National Botanical Research InstituteLucknowIndia