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Mossambicus tilapia (Oreochromis mossambicus) collected from water bodies impacted by urban waste carries extended-spectrum beta-lactamases and integron-bearing gut bacteria

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Abstract

Oreochromis mossambicus (Peters 1852) (Tilapia) is one of the most consumed fish globally. Tilapia thrives well in environments polluted by urban waste, which invariably contain antibiotic-resistant bacteria and antibiotic resistance genes (ARGs). Thus, Tilapia surviving in such polluted environments may serve as a potential source for dissemination of ARGs. To investigate this, we isolated bacterial strains from gut of Tilapia found in polluted rivers and lakes near Pune, India, and studied the prevalence of resistance genes by molecular methods. A total of 91 bacterial strains were obtained, which include fish pathogens and human pathogens such as Aeromonas hydrophila, Klebsiella pneumoniae, E. coli, Serratia marcescens, Enterobacter spp. and Shigella spp. Overall the prevalence of class 1 integrons, class 2 integrons, extended-spectrum beta-lactamases (ESBLs) bla CTX-M, bla SHV and aac(6')-Ib-cr gene was 38%, 24%, 38%, 31% and 31% respectively. Forty-two percent of the Enterobacteriaceae strains carried bla CTX-M gene, which is a common ESBL gene in clinics. The study demonstrates that tilapia found in the polluted waters can serve as reservoirs and an alternative route for human exposure to clinically important ARG-carrying bacteria. The consumption and handling of these fish may pose a potential health risk.

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Acknowledgements

This work was supported by the Dr DS Kothari Postdoctoral Fellowship Scheme by University Grant Commission, India, to SSG; Departmental Research and Development Grant from the Department of Biotechnology,India, and University for Potential Excellence (UPE-II) Phase Program and DBT, India, for providing funds to carry out this work under the MCC project grant no. BT/PR10054/NDB/52/94/2007.

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Author notes

  1. Nachiket P Marathe

    Present address: Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy at the University of Gothenburg, SE-413 46, Göteborg, Sweden

Authors and Affiliations

  1. Microbial Culture Collection, National Centre for Cell Science, Pune, 411 007, India

    Nachiket P Marathe, Swapnil S Gaikwad, Ankita A Vaishampayan, Mandar H Rasane & Yogesh S Shouche

  2. Department of Biotechnology, Savitribai Phule Pune University, Pune, 411 007, India

    Swapnil S Gaikwad & Wasudev N Gade

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  1. Nachiket P Marathe
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  2. Swapnil S Gaikwad
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  3. Ankita A Vaishampayan
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Correspondence to Wasudev N Gade.

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Corresponding editor: GAGANDEEP KANG

[Marathe NP, Gaikwad SS, Vaishampayan AA, Rasane MH, Shouche YS and Gade WN 2016 Mossambicus tilapia (Oreochromis mossambicus) collected from water bodies impacted by urban waste carries extended-spectrum beta-lactamases and integron-bearing gut bacteria. J. Biosci.]

Supplementary materials pertaining to this article are available on the Journal of Biosciences Website.

Nachiket P Marathe and Swapnil S Gaikwad contributed equally to this work.

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Marathe, N.P., Gaikwad, S.S., Vaishampayan, A.A. et al. Mossambicus tilapia (Oreochromis mossambicus) collected from water bodies impacted by urban waste carries extended-spectrum beta-lactamases and integron-bearing gut bacteria. J Biosci 41, 341–346 (2016). https://doi.org/10.1007/s12038-016-9620-2

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  • DOI: https://doi.org/10.1007/s12038-016-9620-2

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