Abstract
The interplay between the host and Human cytomegalovirus (HCMV) plays a pivotal role in the outcome of an infection. HCMV growth in endothelial and epithelial cells requires expression of viral proteins UL128, UL130, and UL131 proteins (UL128-131), of which UL130 is the largest gene and the only one that is not interrupted by introns. Mutation of the C terminus of the UL130 protein causes reduced tropism of endothelial cells (EC). However, very few host factors have been identified that interact with the UL130 protein. In this study, HCMV UL130 protein was shown to directly interact with the human protein Snapin in human embryonic kidney HEK293 cells by Yeast two-hybrid screening, in vitro glutathione S-transferase (GST) pull-down, and co-immunoprecipitation. Additionally, heterologous expression of protein UL130 revealed co-localization with Snapin in the cell membrane and cytoplasm of HEK293 cells using fluorescence confocal microscopy. Furthermore, decreasing the level of Snapin via specific small interfering RNAs decreased the number of viral DNA copies and titer in HCMV-infected U373-S cells. Taken together, these results suggest that Snapin, the pUL130 interacting protein, has a role in modulating HCMV DNA synthesis.
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Abbreviations
- Co-IP:
-
Co-immunoprecipitation
- HCMV:
-
Human cytomegalovirus
- ORF:
-
open reading frame
- PCR:
-
polymerase chain reaction
- siRNAs:
-
small interfering RNAs
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (81171580 and 81171581) and the Outstanding Scientific Fund of Shengjing Hospital.
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Corresponding editor: Shahid Jameel
Corresponding editor: Shahid Jameel
[Wang G, Ren G, Cui X, Lu Z, Ma Y, Qi Y, Huang Y, Liu Z, Sun Z and Ruan Q 2016 Host protein Snapin interacts with human cytomegalovirus pUL130 and affects viral DNA replication. J. Biosci.] DOI 10.1007/s12038-016-9604-2
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Wang, G., Ren, G., Cui, X. et al. Host protein Snapin interacts with human cytomegalovirus pUL130 and affects viral DNA replication. J Biosci 41, 173–182 (2016). https://doi.org/10.1007/s12038-016-9604-2
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DOI: https://doi.org/10.1007/s12038-016-9604-2