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Do seed VLCFAs trigger spongy tissue formation in Alphonso mango by inducing germination?

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Abstract

Spongy tissue is a physiological disorder in Alphonso mango caused by the inception of germination-associated events during fruit maturation on the tree, rendering the fruit inedible. Inter-fruit competition during active fruit growth is a major contributing factor for the disorder which leads to reduced fat content in spongy tissue affected fruits. This study was, therefore, carried out to determine the possible association between seed fats and ST formation. The study of the fat content during fruit growth showed that it increased gradually from 40% fruit maturity. At 70% maturity, however, there was a sudden increase of fat content of whole fruit, leading to acute competition and resulting in differential allocation of resources among developing fruits. As a result, the seed in spongy-tissue-affected mature ripe fruit showed a marked drop in the levels of fats and the two very long chain fatty acids (VLCFAs), tetracosanoic acid and hexacosanoic acid together with an increase of linolenic acid and a fall in oleic acid contents, which are known to be key determinants for the initiation of pre-germination events in seed. Subsequently, a rise in the level of cytokinin and gibberellins in ST seed associated with a fall in abscisic acid level clearly signalled the onset of germination. Concurrently, a significant reduction in the ratio of linolenic acid/linoleic acid in pulp led to the loss of membrane integrity, cell death and the eventual formation of spongy tissue. Based on the above, it is concluded that a significant reduction in the biosynthesis of VLCFAs in seeds during fruit growth might trigger pre-germination events followed by a cascade of biochemical changes in the pulp, leading to lipid peroxidation and membrane injury in pulp culminating in ST development. Thus, this study presents crucial experimental evidence to highlight the critical role played by VLCFAs in inducing ST formation in Alphonso mango during the pre-harvest phase of fruit growth.

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Acknowledgements

We thank the Director, Indian Institute of Horticultural Research, Bangalore for providing access to facilities. Grateful thanks are due to Dr KK Upreti, Principal Scientist, and Mr HL Jayaram, Chief Technical Officer, for help in the analysis of seed hormones.

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Authors and Affiliations

  1. Division of Plant Physiology and Biochemistry, Indian Institute of Horticultural Research, Bangalore, India

    Seshadri Shivashankar, Manoharan Sumathi & Tapas Kumar Roy

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  1. Seshadri Shivashankar
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  2. Manoharan Sumathi
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  3. Tapas Kumar Roy
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Correspondence to Seshadri Shivashankar.

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Corresponding editor: Man Mohan Johri

[Shivashankar S, Sumathi M and Roy TK 2015 Do seed VLCFAs trigger spongy tissue formation in Alphonso mango by inducing germination? J. Biosci. 40 1–13] DOI 10.1007/s12038-015-9515-7

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Shivashankar, S., Sumathi, M. & Roy, T.K. Do seed VLCFAs trigger spongy tissue formation in Alphonso mango by inducing germination?. J Biosci 40, 375–387 (2015). https://doi.org/10.1007/s12038-015-9515-7

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  • DOI: https://doi.org/10.1007/s12038-015-9515-7

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