Article

Journal of Biosciences

, Volume 35, Issue 4, pp 557-564

Proteomic analysis of cell lines to identify the irinotecan resistance proteins

  • Xing-Chen PengAffiliated withDepartment of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University
  • , Feng-Ming GongAffiliated withDepartment of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University
  • , Meng WeiAffiliated withDepartment of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University
  • , Xi ChenAffiliated withDepartment of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University
  • , Ye ChenAffiliated withDepartment of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University
  • , Ke ChengAffiliated withDepartment of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University
  • , Feng GaoAffiliated withDepartment of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University
  • , Feng XuAffiliated withDepartment of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University
  • , Feng BiAffiliated withDepartment of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University
    • , Ji-Yan LiuAffiliated withDepartment of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University Email author 

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Abstract

Chemotherapeutic drug resistance is a frequent cause of treatment failure in colon cancer patients. Several mechanisms have been implicated in drug resistance. However, they are not sufficient to exhaustively account for this resistance emergence. In this study, two-dimensional gel electrophoresis (2-DE) and the PDQuest software analysis were applied to compare the differential expression of irinotecan-resistance-associated protein in human colon adenocarcinoma LoVo cells and irinotecan-resistant LoVo cells (LoVo/irinotecan). The differential protein dots were excised and analysed by ESI-Q-TOF mass spectrometry (MS). Fifteen proteins were identified, including eight proteins with decreased expression and seven proteins with increased expression. The identified known proteins included those that function in diverse biological processes such as cellular transcription, cell apoptosis, electron transport/redox regulation, cell proliferation/differentiation and retinol metabolism pathways. Identification of such proteins could allow improved understanding of the mechanisms leading to the acquisition of chemoresistance.

Keywords

Colon cancer 2-DE drug resistance irinotecan proteomics