Journal of Biosciences

, Volume 33, Issue 2, pp 279–287

Nano-composition of riboflavin-nafion functional film and its application in biosensing


  • S. Rezaei-Zarchi
    • Institute of Biochemistry and BiophysicsUniversity of Tehran
    • Department of BiologyPayam-e-Noor University
    • Institute of Biochemistry and BiophysicsUniversity of Tehran
  • A. Javed
    • Department of BiochemistryUniversity of Agriculture
  • A. Barzegar
    • Institute of Biochemistry and BiophysicsUniversity of Tehran
  • S. Ahmadian
    • Institute of Biochemistry and BiophysicsUniversity of Tehran
  • A. Bayandori-Moghaddam
    • Department of Chemistry, Faculty of SciencesUniversity of Tehran

DOI: 10.1007/s12038-008-0045-4

Cite this article as:
Rezaei-Zarchi, S., Saboury, A.A., Javed, A. et al. J Biosci (2008) 33: 279. doi:10.1007/s12038-008-0045-4


A novel nafion-riboflavin membrane was constructed and characterized by the scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible spectroscopy and cyclic voltammetric techniques. The estimated average diameter of the designed nanoparticles was about 60 nm. The functional membrane showed a quasi-reversible electrochemical behaviour with a formal potential of −562 ± 5 mV (vs Ag/AgCl) on the gold electrode. Some electrochemical parameters were estimated, indicating that the system has good and stable electron transfer properties. Moreover, horseradish peroxidase (HRP) was immobilized on the riboflavin-nafion functional membrane. The electrochemical behaviour of HRP was quasi-reversible with a formal potential of 80 ± 5 mV (vs Ag/AgCl). The HRP in the film exhibited good catalytic activity towards the reduction of H2O2. It shows a linear dependence of its cathodic peak current on the concentration of H2O2, ranging from 10 to 300 µM.


Biosensorfunctional membranehorseradish peroxidasenanoparticles

Abbreviations used


horseradish peroxidase


nafion perfluorosulphonated ion-exchange resin


scanning electron microscopy


transmission electron microscopy

Copyright information

© Indian Academy of Sciences 2008