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Nano-composition of riboflavin-nafion functional film and its application in biosensing

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Abstract

A novel nafion-riboflavin membrane was constructed and characterized by the scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible spectroscopy and cyclic voltammetric techniques. The estimated average diameter of the designed nanoparticles was about 60 nm. The functional membrane showed a quasi-reversible electrochemical behaviour with a formal potential of −562 ± 5 mV (vs Ag/AgCl) on the gold electrode. Some electrochemical parameters were estimated, indicating that the system has good and stable electron transfer properties. Moreover, horseradish peroxidase (HRP) was immobilized on the riboflavin-nafion functional membrane. The electrochemical behaviour of HRP was quasi-reversible with a formal potential of 80 ± 5 mV (vs Ag/AgCl). The HRP in the film exhibited good catalytic activity towards the reduction of H2O2. It shows a linear dependence of its cathodic peak current on the concentration of H2O2, ranging from 10 to 300 µM.

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Abbreviations

HRP:

horseradish peroxidase

nafion:

nafion perfluorosulphonated ion-exchange resin

SEM:

scanning electron microscopy

TEM:

transmission electron microscopy

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    S. Rezaei-Zarchi, A. A. Saboury, A. Barzegar & S. Ahmadian

  2. Department of Biology, Payam-e-Noor University, Yazd, Iran

    S. Rezaei-Zarchi

  3. Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan

    A. Javed

  4. Department of Chemistry, Faculty of Sciences, University of Tehran, Tehran, Iran

    A. Bayandori-Moghaddam

Authors
  1. S. Rezaei-Zarchi
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  2. A. A. Saboury
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  3. A. Javed
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  4. A. Barzegar
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  5. S. Ahmadian
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  6. A. Bayandori-Moghaddam
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Correspondence to A. A. Saboury.

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Rezaei-Zarchi, S., Saboury, A.A., Javed, A. et al. Nano-composition of riboflavin-nafion functional film and its application in biosensing. J Biosci 33, 279–287 (2008). https://doi.org/10.1007/s12038-008-0045-4

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  • DOI: https://doi.org/10.1007/s12038-008-0045-4

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