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Transgenic Mouse Milk Expressing Human Bile Salt-Stimulated Lipase Improves the Survival and Growth Status of Premature Mice

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Abstract

The lactating human mammary gland and the pancreas both produce bile salt-stimulated lipase (BSSL), a lipolytic enzyme acting on a wide range of substrates, including triglyceride, cholesterol esters, and fat-soluble vitamins esters. Breast milk BSSL has a particularly important role in the digestion of milk fat by newborn infants. We report the generation of transgenic mice that harbored a human BSSL gene controlled by a mammary gland-specific promoter. BSSL levels in transgenic mouse milk were raised to 376.8 μg/ml, corresponding to an activity of 9.15 U/ml. Premature wild-type neonates nursed by transgenic dams exhibited significantly higher survival rate than did the control neonates nursed by wild dams (95 vs. 83.3 % and, P < 0.05). They also showed 43.8 % greater body weight gain and 33.3 % lesser fecal crude fat levels than did the controls. This study provides significant evidence that increased levels of BSSL in milk may reduce mortality and improve the growth and fat absorption in premature mice during neonatal development.

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Acknowledgments

This work was supported by National Transgenic Breeding Program of China (2009ZX08007-001B) and (2009ZX08009-139B), Bengbu Medical College (Bykf13A01), and Funds for Distinguished Young Scholar of Anhui Province, China (2013SQRL050ZD).

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Authors and Affiliations

  1. Department of Biology Science, Bengbu Medical College, Bengbu, 233030, Anhui, China

    Yuanyuan Wang

  2. State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

    Yuanyuan Wang, Zheya Sheng, Yuhang Wang, Qinghe Li, Yunping Dai, Yaofeng Zhao & Ning Li

  3. Division of Computational Genetics, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden

    Zheya Sheng

  4. Institute of Cardiovascular Sciences, Health Science Center, Peking University, Beijing, 100083, China

    Yuhui Wang & George Liu

  5. Shun Da Kang Agricultural Science and Technology Development Co., Ltd., Suzhou, 215000, China

    Yu Gao

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  1. Yuanyuan Wang
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  2. Zheya Sheng
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  3. Yuhang Wang
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  4. Qinghe Li
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  5. Yu Gao
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  7. Yunping Dai
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  8. George Liu
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  9. Yaofeng Zhao
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  10. Ning Li
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Correspondence to Ning Li.

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Yuanyuan Wang and Zheya Sheng have contributed equally to this work.

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Wang, Y., Sheng, Z., Wang, Y. et al. Transgenic Mouse Milk Expressing Human Bile Salt-Stimulated Lipase Improves the Survival and Growth Status of Premature Mice. Mol Biotechnol 57, 287–297 (2015). https://doi.org/10.1007/s12033-014-9822-5

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  • DOI: https://doi.org/10.1007/s12033-014-9822-5

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