Research

Molecular Biotechnology

, Volume 54, Issue 2, pp 609-622

Overexpression of Camellia sinensis Thaumatin-Like Protein, CsTLP in Potato Confers Enhanced Resistance to Macrophomina phaseolina and Phytophthora infestans Infection

  • Karan AcharyaAffiliated withDivision of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology
  • , Awadhesh K. PalAffiliated withDivision of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology
  • , Arvind GulatiAffiliated withHill Area Tea Science Division, CSIR-Institute of Himalayan Bioresource Technology
  • , Sanjay KumarAffiliated withDivision of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology
  • , Anil K. SinghAffiliated withDivision of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology
  • , Paramvir S. AhujaAffiliated withDivision of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology Email author 

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Abstract

Thaumatin-like proteins (TLPs), a class of pathogenesis related proteins are induced in response to pathogens and exhibit antifungal property when overexpressed in transgenic plants. In the present study, we have raised transgenic potato plants overexpressing TLP gene of Camellia sinensis (CsTLP). Fungal resistance assays of transgenic potato elucidated the potential role of CsTLP in imparting tolerance to fungal pathogens, Macrophomina phaseolina (necrotrophic) and Phytophthora infestans (hemi-biotrophic). Transgenic tubers with higher resistance to M. phaseolina, showed a concomitant and significant increase in transcripts of StPAL, StLOX, and StTLP genes involved in phenylpropanoid, lipoxygenase, and general defense response pathway, respectively after infection. Importantly, leaves of CsTLP transgenic lines inoculated with P. infestans spores under in vitro conditions also showed a resistant phenotype. The resistant phenotype recorded for the two important fungal pathogens by CsTLP transgenic potato plants is remarkable, since no effective control methods and no resistant cv. against M. phaseolina has been identified so far in potato.

Keywords

Macrophomina phaseolina Phytophthora infestans Thaumatin-like protein Transgenic potato