Molecular Biotechnology

, Volume 54, Issue 2, pp 609–622

Overexpression of Camellia sinensis Thaumatin-Like Protein, CsTLP in Potato Confers Enhanced Resistance to Macrophomina phaseolina and Phytophthora infestans Infection

Authors

  • Karan Acharya
    • Division of BiotechnologyCSIR-Institute of Himalayan Bioresource Technology
  • Awadhesh K. Pal
    • Division of BiotechnologyCSIR-Institute of Himalayan Bioresource Technology
  • Arvind Gulati
    • Hill Area Tea Science DivisionCSIR-Institute of Himalayan Bioresource Technology
  • Sanjay Kumar
    • Division of BiotechnologyCSIR-Institute of Himalayan Bioresource Technology
  • Anil K. Singh
    • Division of BiotechnologyCSIR-Institute of Himalayan Bioresource Technology
    • Division of BiotechnologyCSIR-Institute of Himalayan Bioresource Technology
Research

DOI: 10.1007/s12033-012-9603-y

Cite this article as:
Acharya, K., Pal, A.K., Gulati, A. et al. Mol Biotechnol (2013) 54: 609. doi:10.1007/s12033-012-9603-y

Abstract

Thaumatin-like proteins (TLPs), a class of pathogenesis related proteins are induced in response to pathogens and exhibit antifungal property when overexpressed in transgenic plants. In the present study, we have raised transgenic potato plants overexpressing TLP gene of Camellia sinensis (CsTLP). Fungal resistance assays of transgenic potato elucidated the potential role of CsTLP in imparting tolerance to fungal pathogens, Macrophomina phaseolina (necrotrophic) and Phytophthora infestans (hemi-biotrophic). Transgenic tubers with higher resistance to M. phaseolina, showed a concomitant and significant increase in transcripts of StPAL, StLOX, and StTLP genes involved in phenylpropanoid, lipoxygenase, and general defense response pathway, respectively after infection. Importantly, leaves of CsTLP transgenic lines inoculated with P. infestans spores under in vitro conditions also showed a resistant phenotype. The resistant phenotype recorded for the two important fungal pathogens by CsTLP transgenic potato plants is remarkable, since no effective control methods and no resistant cv. against M. phaseolina has been identified so far in potato.

Keywords

Macrophomina phaseolina Phytophthora infestans Thaumatin-like protein Transgenic potato

Abbreviations

PR

Pathogenesis related

TLP

Thaumatin-like protein

PAL

Phenyl-alanine ammonia lyase

LOX

Lipoxygenase

CsTLP

Camellia sinensis thaumatin-like protein

Supplementary material

12033_2012_9603_MOESM1_ESM.doc (308 kb)
Fig. S1: Alignment of amino acid sequence of Nicotiana tabacum osmotin (Nt-Osmotin, AAA34089) with C. sinensis thaumatin-like protein (CsTLP, ABE01396) using ClustalW. (DOC 308 kb). Supplementary material 1 (DOC 308 kb)

Copyright information

© Springer Science+Business Media New York 2012