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CKC: Isolation of Nucleic Acids from a Diversity of Plants Using CTAB and Silica Columns

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Abstract

To assay for viruses in plant samples, we required a method for nucleic acid isolation that is rapid, simple, and applicable to the widest possible variety of plants. A protocol for isolation of total nucleic acid (TNA) was developed by combining common CTAB methods with silica spin columns. We report data on TNA purity and RNA quality from over 30 plant genera representing 25 families. Measurements showed that RNA is of high quality, and one-step RT-PCR was successfully performed on all samples. The protocol can be completed in less than 2 h.

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Acknowledgments

This project was supported by USDA-ARS Research Project No. 1230-22000-022-00D and the National Research Initiative Competitive Grants Program Grant No. 2009-55605-05023 from the National Institute of Food and Agriculture. The authors thank R. L. Jordan and M. A. Guaragna for their gift of the IFBV primer set.

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Authors and Affiliations

  1. USDA-ARS, United States National Arboretum, Floral and Nursery Plants Research Unit, B-010A, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA

    David C. Henderson & John Hammond

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  1. David C. Henderson
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  2. John Hammond
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Correspondence to David C. Henderson.

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Henderson, D.C., Hammond, J. CKC: Isolation of Nucleic Acids from a Diversity of Plants Using CTAB and Silica Columns. Mol Biotechnol 53, 109–117 (2013). https://doi.org/10.1007/s12033-012-9494-y

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