Abstract
Autotaxin (ATX) as an important tumor cell motility-stimulating factor is upregulated in many different types of cancer. ATX, a member of the ectonucleotide pyrophosphatase and phosphodiesterase family of enzymes, possesses lysophospholipase D activity which hydrolyzes lysophosphatidylcholine to generate the potent tumor growth factor and mitogen lysophosphatidic acid (LPA). LPA acts on specific G-protein-coupled receptors, thereby regulating cell growth, migration, and survival. This study aimed to investigate the differences in gene expression pattern of ATX between cancerous and adjacent normal tissue of human renal cell carcinoma (RCC) and bladder carcinoma (BC) and find the correlation between ATX expression and clinicopathological features of both of these carcinomas. Both the RCC and BC tissues and with the adjacent normal tissues were collected. Immunohistochemistry and Western blotting analysis were used to detect the extent of ATX expression in all of these samples. Immunohistochemistry and Western blot analysis revealed that expression of ATX protein in carcinoma tissues is significantly higher than that in the adjacent normal tissues. Immunohistochemistry analysis showed that ATX is localized in cytoplasm. Western blotting analysis showed that ATX protein is expressed in both RCC and BC, and the expression levels were 69.5 and 48.0 %, respectively, higher in RCC and BC carcinoma tissue samples than in the adjacent normal tissues, which is consistent with the results of immunohistochemistry study. Thus, this study provided the evidence that ATX is highly expressed in both RCC and BC. Further research can be done to identify the diagnosis and treatment significance of both these carcinomas.
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The work was supported by the Grant 81071685 from the National Natural Science Foundation of China.
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The authors declare no conflicts of interest in relation to the publication of this study. The authors have full control of all of the primary data and agree to allow the journal to review the data if needed.
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This study was approved by the medical ethics committee of the Xiangya Second Hospital of Central South University hospital.
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For the use of all clinical materials for research purposes, prior written patient’s consent and approval from the Institute Research Ethics Committee were obtained.
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Aihua Xu and Md. Ahsanul Kabir Khan contributed equally to this work and are considered as first author.
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Xu, A., Ahsanul Kabir Khan, M., Chen, F. et al. Overexpression of autotaxin is associated with human renal cell carcinoma and bladder carcinoma and their progression. Med Oncol 33, 131 (2016). https://doi.org/10.1007/s12032-016-0836-7
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DOI: https://doi.org/10.1007/s12032-016-0836-7