Medical Oncology

, 31:785

MicroRNA-205 suppresses proliferation and promotes apoptosis in laryngeal squamous cell carcinoma

Original Paper

DOI: 10.1007/s12032-013-0785-3

Cite this article as:
Tian, L., Zhang, J., Ge, J. et al. Med Oncol (2014) 31: 785. doi:10.1007/s12032-013-0785-3

Abstract

MicroRNAs were reported to be involved in the modulation of tumor development. The aim of our study was to investigate the effect of miR-205 on proliferation and apoptosis of laryngeal squamous cell carcinoma (LSCC) and seek associations between miR-205 and Bcl-2 using in vitro and in vivo methods. Real-time qPCR was used to analyze the expression of miR-205 in LSCC samples and Hep-2 cell line. Apoptosis, cell cycle, and proliferation (MTT) assays were performed to test the apoptosis and proliferation of LSCC cells after miR-205 transfection. Bcl-2 expression in cells was assessed with Western blotting. The tumorigenicity of LSCC cells was evaluated in nude mice model. MiR-205 was significantly down-regulated in LSCC tissues compared to adjacent normal tissues. Lower expression of miR-205 was indicated to be statistically related with advanced clinical stage and T3–4 grades. We found that restoration of miR-205 down-regulated the proliferative markers of dihydrofolate reductase and proliferating cell nuclear antigen and apoptotic regulator of Bcl-2. The findings in vitro and in vivo showed miR-205 could suppress cell proliferation and induce cell apoptosis. In addition, Bcl-2 was identified as one of the direct targets of miR-205 in LSCC cells. These results suggest that miR-205 may play as a tumor suppressor in LSCC, probably by targeting Bcl-2 and serve as a potential target for therapeutic intervention.

Keywords

Laryngeal squamous cell carcinoma MiR-205 Bcl-2 Proliferation Apoptosis 

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Linli Tian
    • 1
  • Jiarui Zhang
    • 1
  • Jingchun Ge
    • 1
  • Hui Xiao
    • 1
  • Jianguang Lu
    • 1
  • Songbin Fu
    • 2
  • Ming Liu
    • 1
  • Yanan Sun
    • 1
  1. 1.Department of Otolaryngology, Head and Neck Surgery, The Second Affiliated HospitalHarbin Medical UniversityHarbinChina
  2. 2.Laboratory of Medical GeneticsHarbin Medical UniversityHarbinChina

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