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Prediction and identification of B cell epitopes derived from EWS/FLI-l fusion protein of Ewing’s sarcoma

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Abstract

To predict B cell epitope of Ewing’s sarcoma EWS/FLI-l fusion protein and to analyze its antigenicity and immunogenicity. Comprehensive algorithms were applied to predict the possible B cell epitopes of EWS/FLI-l fusion protein. High-performance liquid chromatography (HPLC) and mass spectrometry (MS) analysis were performed to identify the synthesized epitope peptides, ELISA assays and Western blot to detect the antigenicity, and the immunogenicity of epitope peptides. Three B cell epitopes were screened out, and HPLC and MS analysis confirmed all three synthesized epitope peptides were demandable. ELISA assays verified all three epitope peptides could prime intense antigen–antibody reaction and induce ideal antibody titers after immunization to the New Zealand white rabbit. However, Western blot confirmed that antiserum of one of these epitope peptides could not recognize EWS/FLI-1 protein. Two B cell epitopes, PQDGNKPTETSQPQ and DPDEVARRWGQRKS, derived from EWS/FLI-l protein, are identified to have potential antigenicity and immunogenicity.

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Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (grants No. 30760254 and 81060221), the Natural Science Foundation of Jiangxi Province (grants No. 2009GQY0204), and the Education Department of Jiangxi Province(grants No. GJJ08076).

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Authors and Affiliations

  1. Department of Orthopaedics, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People’s Republic of China

    Huiwen Liu, Jiaquan Luo, Wenzhao Chen, Xiang Liao, Min Dai, Yong Shu & Kai Cao

  2. Department of Child Care, Jiangxi Maternal and Child Health Hospital, Nanchang, 330006, People’s Republic of China

    Lu Huang

  3. Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People’s Republic of China

    Zhanmin Zhang

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  1. Huiwen Liu
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  2. Lu Huang
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  4. Wenzhao Chen
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  6. Xiang Liao
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  7. Min Dai
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  9. Kai Cao
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Corresponding author

Correspondence to Kai Cao.

Additional information

Huiwen Liu and Lu Huang are equally contributed to this paper.

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Liu, H., Huang, L., Luo, J. et al. Prediction and identification of B cell epitopes derived from EWS/FLI-l fusion protein of Ewing’s sarcoma. Med Oncol 29, 3421–3430 (2012). https://doi.org/10.1007/s12032-012-0243-7

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  • DOI: https://doi.org/10.1007/s12032-012-0243-7

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