Immunohistochemical detection of HSP27 and hnRNP K as prognostic and predictive biomarkers for colorectal cancer Authors
First Online: 23 August 2011 Received: 25 July 2011 Accepted: 27 July 2011 DOI:
Cite this article as: Wang, F., Zhang, P., Shi, C. et al. Med Oncol (2012) 29: 1780. doi:10.1007/s12032-011-0037-3 Abstract
The present study was aimed at evaluating the expression of heat shock protein 27 (HSP27) and heterogeneous nuclear ribonucleoprotein K (hnRNP K), two potential biomarkers of many cancers, in colorectal cancer (CRC) and their clinical significance. Expression of HSP27 and hnRNP K were investigated by immunohistochemistry (IHC) in a series of tissue microarrays containing 175 primary colorectal cancers and their corresponding normal mucosa samples and matched with clinicopathological features and patient survival. HSP27 and hnRNP K displayed more frequent strong immunoreactivity in primary colorectal tumor samples compared with adjacent non-cancer tissue (
P < 0.001). Increased cytoplasmic expression of HSP27 and hnRNP K were associated with tumor location ( P = 0.032 and P < 0.001, respectively), poorer overall survival ( P = 0.004 and P = 0.02, respectively) and to an unfavorable prognosis for CRC patients in multivariate analysis ( P = 0.019 and P = 0.01, respectively). Their overexpression combination identified a subset of patients with definitively worse prognosis than any other combination ( P < 0.001). Overexpression of HSP27 and hnRNPK were independent markers of poor prognosis, and their combination predicted definitively adverse outcomes in CRC patients. Keywords Colorectal cancer Immunohistochemistry Tissue microarray Prognosis Heat shock protein 27 (HSP27) Heterogeneous nuclear ribonucleoprotein K (hnRNP K) Abbreviations CRC
Heat shock protein 27
Heterogeneous nuclear ribonucleoprotein K
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