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Immunohistochemical detection of HSP27 and hnRNP K as prognostic and predictive biomarkers for colorectal cancer

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Abstract

The present study was aimed at evaluating the expression of heat shock protein 27 (HSP27) and heterogeneous nuclear ribonucleoprotein K (hnRNP K), two potential biomarkers of many cancers, in colorectal cancer (CRC) and their clinical significance. Expression of HSP27 and hnRNP K were investigated by immunohistochemistry (IHC) in a series of tissue microarrays containing 175 primary colorectal cancers and their corresponding normal mucosa samples and matched with clinicopathological features and patient survival. HSP27 and hnRNP K displayed more frequent strong immunoreactivity in primary colorectal tumor samples compared with adjacent non-cancer tissue (P < 0.001). Increased cytoplasmic expression of HSP27 and hnRNP K were associated with tumor location (P = 0.032 and P < 0.001, respectively), poorer overall survival (P = 0.004 and P = 0.02, respectively) and to an unfavorable prognosis for CRC patients in multivariate analysis (P = 0.019 and P = 0.01, respectively). Their overexpression combination identified a subset of patients with definitively worse prognosis than any other combination (P < 0.001). Overexpression of HSP27 and hnRNPK were independent markers of poor prognosis, and their combination predicted definitively adverse outcomes in CRC patients.

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Abbreviations

CRC:

Colorectal cancer

IHC:

Immunohistochemistry

TMA:

Tissue microarray

HSP27:

Heat shock protein 27

hnRNP K:

Heterogeneous nuclear ribonucleoprotein K

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Acknowledgments

This study was supported by grants from the Ph.D. Grant of Shanghai JiaoTong University School of Medicine (No.BXJ201137), the Major Basic Research Program of Shanghai (No. 07DZ19505), and the National 973 Basic Research Program of China (No. 2008CB517403).

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Authors and Affiliations

  1. Department of Surgery, The Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, 600 Yishan Road, Shanghai, 200233, China

    Feng Wang, Peng Zhang, Chenzhang Shi, Yongzhi Yang & Huanlong Qin

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  1. Feng Wang
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  2. Peng Zhang
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  3. Chenzhang Shi
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Correspondence to Huanlong Qin.

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Wang, F., Zhang, P., Shi, C. et al. Immunohistochemical detection of HSP27 and hnRNP K as prognostic and predictive biomarkers for colorectal cancer. Med Oncol 29, 1780–1788 (2012). https://doi.org/10.1007/s12032-011-0037-3

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  • DOI: https://doi.org/10.1007/s12032-011-0037-3

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