Original paper

Medical Oncology

, Volume 28, Issue 1, pp 99-107

Oxymatrine diminishes the side population and inhibits the expression of β-catenin in MCF-7 breast cancer cells

  • Ying ZhangAffiliated withGuang An Men Hospital of China Academy of Chinese Medical Sciences
  • , Bingkui PiaoAffiliated withGuang An Men Hospital of China Academy of Chinese Medical Sciences
  • , Yong ZhangAffiliated withShaanxi University of Chinese Medicine
  • , Baojin HuaAffiliated withGuang An Men Hospital of China Academy of Chinese Medical Sciences
  • , Wei HouAffiliated withGuang An Men Hospital of China Academy of Chinese Medical Sciences
  • , Weiru XuAffiliated withGuang An Men Hospital of China Academy of Chinese Medical Sciences
  • , Xin QiAffiliated withGuang An Men Hospital of China Academy of Chinese Medical Sciences
  • , Xiaoyun ZhuAffiliated withGuang An Men Hospital of China Academy of Chinese Medical Sciences
  • , Yingxia PeiAffiliated withGuang An Men Hospital of China Academy of Chinese Medical Sciences

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Cancer stem cells (CSCs) play a critical role in both cancer initiation and relapse as they are resistant to most cytotoxic agents and able to proliferate indefinitely. The plant alkaloid oxymatrine has many biological activities including the ability to induce cell cycle arrest and apoptosis, which makes it a potentially useful agent for targeting cancer cells. In order to determine whether it has beneficial pharmacological properties to eradicate CSCs, we analyzed the effects of oxymatrine on MCF-7 breast cancer cells. Cancer stem-like cells’ (side population, SP) identification and sorting were performed. The inhibitory effect of oxymatrine was evaluated on the sorted SP and non-SP cells. The results indicated that oxymatrine caused a dose-dependent reduction in the proliferation of MCF-7 cells and a decrease in SP cells. Wnt/β-catenin signaling pathway was also examined by analyzing the expression of total β-catenin and phosphorylated β-catenin in cytoplasm, and the results showed that the growth inhibitory effects of oxymatrine treatment on MCF-7 cells may be due to the inhibition of SP and Wnt/β-catenin signaling pathway. Further work is warranted to explore whether oxymatrine may be a useful novel therapeutic drug for targeting breast CSCs.

Keywords

Oxymatrine MCF-7 Breast cancer Cancer stem cell Side population β-Catenin