Skip to main content
SpringerLink
Log in

The Tau-Induced Reduction of mRNA Levels of Kv Channels in Human Neuroblastoma SK-N-SH Cells

  • Published:
Journal of Molecular Neuroscience Aims and scope Submit manuscript

Abstract

Previous findings indicated that microtubule-binding protein tau and voltage-gated K+ (Kv) channels exhibit a regulatory role in cell proliferation. However, the possible interaction of tau with Kv channels remained obscure. In this report, transfection of tau plasmids into human neuroblastoma SK-N-SH cells caused a significant reduction in the messenger RNA (mRNA) levels of several Kv channels, including Kv2.1, Kv3.1, Kv5.1, Kv9.2, and KCNH4. Correspondingly, the Kv currents recorded using patch-clamp techniques were substantially declined in the tau-transfected SK-N-SH cells. Moreover, tau induction and treatment with the Kv channel blocker TEA (tetraethylammonium) were able to improve proliferation rates of SK-N-SH cells by 43.1 and 66.2 %, respectively. These data suggested that the tau-mediated alteration of Kv channels could be involved in its action on neural proliferation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Arcangeli A, Bianchi L, Becchetti A, Faravelli L, Coronnello M, Mini E, Olivotto M, Wanke E (1995) A novel inward-rectifying K+ current with a cell-cycle dependence governs the resting potential of mammalian neuroblastoma cells. J Physiol 489(Pt 2):455–471

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Avila J, Lucas JJ, Perez M, Hernandez F (2004) Role of tau protein in both physiological and pathological conditions. Physiol Rev 84(2):361–384. doi:10.1152/physrev.00024.2003

    Article  CAS  PubMed  Google Scholar 

  • Brandt R, Leger J, Lee G (1995) Interaction of tau with the neural plasma membrane mediated by tau’s amino-terminal projection domain. J Cell Biol 131(5):1327–1340

    Article  CAS  PubMed  Google Scholar 

  • Burg ED, Remillard CV, Yuan JX (2006) K+ channels in apoptosis. J Membr Biol 209(1):3–20. doi:10.1007/s00232-005-0838-4

    Article  CAS  PubMed  Google Scholar 

  • Chetty CS, Vemuri MC, Reddy GR, Suresh C (2007) Protective effect of 17-beta-estradiol in human neurocellular models of lead exposure. Neurotoxicology 28(2):396–401. doi:10.1016/j.neuro.2006.03.012

    Article  CAS  PubMed  Google Scholar 

  • Drechsel DN, Hyman AA, Cobb MH, Kirschner MW (1992) Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau. Mol Biol Cell 3(10):1141–1154

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Ferreira A, Caceres A (1991) Estrogen-enhanced neurite growth: evidence for a selective induction of Tau and stable microtubules. J Neurosci 11(2):392–400

    CAS  PubMed  Google Scholar 

  • Fieber LA, Gonzalez DM, Wallace MR, Muir D (2003) Delayed rectifier K currents in NF1 Schwann cells. Pharmacological block inhibits proliferation. Neurobiol Dis 13(2):136–146

    Article  CAS  PubMed  Google Scholar 

  • Guise S, Braguer D, Remacle-Bonnet M, Pommier G, Briand C (1999) Tau protein is involved in the apoptotic process induced by anti-microtubule agents on neuroblastoma cells. Apoptosis 4(1):47–58

    Article  CAS  PubMed  Google Scholar 

  • Hong XP, Peng CX, Wei W, Tian Q, Liu YH, Yao XQ, Zhang Y, Cao FY, Wang Q, Wang JZ (2010) Essential role of tau phosphorylation in adult hippocampal neurogenesis. Hippocampus 20(12):1339–1349. doi:10.1002/hipo.20712

    Article  CAS  PubMed  Google Scholar 

  • Ikeda H, Taira N, Hara F, Fujita T, Yamamoto H, Soh J, Toyooka S, Nogami T, Shien T, Doihara H, Miyoshi S (2010) The estrogen receptor influences microtubule-associated protein tau (MAPT) expression and the selective estrogen receptor inhibitor fulvestrant downregulates MAPT and increases the sensitivity to taxane in breast cancer cells. Breast Cancer Res 12(3):R43. doi:10.1186/bcr2598

    Article  PubMed Central  PubMed  Google Scholar 

  • Lang F, Foller M, Lang KS, Lang PA, Ritter M, Gulbins E, Vereninov A, Huber SM (2005) Ion channels in cell proliferation and apoptotic cell death. J Membr Biol 205(3):147–157. doi:10.1007/s00232-005-0780-5

    Article  CAS  PubMed  Google Scholar 

  • Lee VM, Balin BJ, Otvos L Jr, Trojanowski JQ (1991) A68: a major subunit of paired helical filaments and derivatized forms of normal Tau. Science 251(4994):675–678

    Article  CAS  PubMed  Google Scholar 

  • Moschner K, Sundermann F, Meyer H, da Graca AP, Appel N, Paululat A, Bakota L, Brandt R (2014) RNA protein granules modulate tau isoform expression and induce neuronal sprouting. J Biol Chem 289(24):16814–16825. doi:10.1074/jbc.M113.541425

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Ouadid-Ahidouch H, Chaussade F, Roudbaraki M, Slomianny C, Dewailly E, Delcourt P, Prevarskaya N (2000) KV1.1 K+ channels identification in human breast carcinoma cells: involvement in cell proliferation. Biochem Biophys Res Commun 278(2):272–277. doi:10.1006/bbrc.2000.3790

    Article  CAS  PubMed  Google Scholar 

  • Perez M, Santa-Maria I, Gomez de Barreda E, Zhu X, Cuadros R, Cabrero JR, Sanchez-Madrid F, Dawson HN, Vitek MP, Perry G, Smith MA, Avila J (2009) Tau—an inhibitor of deacetylase HDAC6 function. J Neurochem 109(6):1756–1766. doi:10.1111/j.1471-4159.2009.06102.x

    Article  CAS  PubMed  Google Scholar 

  • Rouzaire-Dubois B, Dubois JM (1990) Tamoxifen blocks both proliferation and voltage-dependent K+ channels of neuroblastoma cells. Cell Signal 2(4):387–393

    Article  CAS  PubMed  Google Scholar 

  • Rouzier R, Rajan R, Wagner P, Hess KR, Gold DL, Stec J, Ayers M, Ross JS, Zhang P, Buchholz TA, Kuerer H, Green M, Arun B, Hortobagyi GN, Symmans WF, Pusztai L (2005) Microtubule-associated protein tau: a marker of paclitaxel sensitivity in breast cancer. Proc Natl Acad Sci U S A 102(23):8315–8320. doi:10.1073/pnas.0408974102

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Schoenfeld TA, Obar RA (1994) Diverse distribution and function of fibrous microtubule-associated proteins in the nervous system. Int Rev Cytol 151:67–137

    Article  CAS  PubMed  Google Scholar 

  • Vest RS, Pike CJ (2013) Gender, sex steroid hormones, and Alzheimer’s disease. Horm Behav 63(2):301–307. doi:10.1016/j.yhbeh.2012.04.006

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Witman GB, Cleveland DW, Weingarten MD, Kirschner MW (1976) Tubulin requires tau for growth onto microtubule initiating sites. Proc Natl Acad Sci U S A 73(11):4070–4074

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Wonderlin WF, Strobl JS (1996) Potassium channels, proliferation and G1 progression. J Membr Biol 154(2):91–107

    Article  CAS  PubMed  Google Scholar 

  • Wu WK, Li GR, Wong HP, Hui MK, Tai EK, Lam EK, Shin VY, Ye YN, Li P, Yang YH, Luo JC, Cho CH (2006) Involvement of Kv1.1 and Nav1.5 in proliferation of gastric epithelial cells. J Cell Physiol 207(2):437–444. doi:10.1002/jcp.20576

    Article  CAS  PubMed  Google Scholar 

  • Yi BA, Minor DL Jr, Lin YF, Jan YN, Jan LY (2001) Controlling potassium channel activities: interplay between the membrane and intracellular factors. Proc Natl Acad Sci U S A 98(20):11016–11023. doi:10.1073/pnas.191351798

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the grants from the research project of MOE of PRC [BZY13030].

Author information

Authors and Affiliations

  1. Graduate Institute of South-Central University for Nationalities, Wuhan, 430074, China

    Xi-Mu Hu & Xiao-Qing Li

  2. Department of Neurobiology, College of Life Sciences, South-Central University for Nationalities, Wuhan, 430074, China

    Xian-Tao Li

Authors
  1. Xi-Mu Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiao-Qing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xian-Tao Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xian-Tao Li.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hu, XM., Li, XQ. & Li, XT. The Tau-Induced Reduction of mRNA Levels of Kv Channels in Human Neuroblastoma SK-N-SH Cells. J Mol Neurosci 58, 306–311 (2016). https://doi.org/10.1007/s12031-015-0683-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12031-015-0683-6

Keywords

Search

Navigation