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Nur77 Was Essential for Neurite Outgrowth and Involved in Schwann Cell Differentiation After Sciatic Nerve Injury

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Abstract

Nur77, together with Nurr1 and NOR-1, constitutes the NR4A subgroup of orphan nuclear receptors and plays critical roles in cell proliferation, differentiation, migration, and apoptosis. Among them, Nur77 is universally well known to contribute to neurite outgrowth. However, information regarding its regulation and possible function in the peripheral nervous system is still limited. In this study, we performed a sciatic nerve injury model in adult rats and detected an increased expression of Nur77 in the sciatic nerve, which was similar to the expression of Oct-6. Immunofluorescence indicated that Nur77 was located in both axons and Schwann cells. In vitro, we observed enhanced expression of Nur77 during the process of both basic fibroblast growth factor (bFGF)-induced Schwann cells differentiation and nerve growth factor (NGF)-induced PC12 cell neurite outgrowth. In vitro and in vivo experiments indicated that inhibiting the function of Nur77 by specific short hairpin RNA could depress Schwann cells myelinization and axons regeneration. Collectively, all these results suggested that upregulation of Nur77 might be involved in Schwann cells differentiation and neurite elongation following sciatic nerve crush.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81171140, No. 81471258), the Colleges and Universities in Natural Science Research Project of Jiangsu Province (No. 13KJB31009), and a project funded by the Priority Academic Program Development of jiangsu Higher Education Institution (PAPD).

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The authors have declared that no conflict of interest exists.

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    Authors and Affiliations

    1. Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

      Weidong Zhang, Yang Liu, Minhao Chen, Shixian Yan, Xingxing Mao, Zhongbing Liu, Weijie Wu, Chen Chen, Xinbao Xu & Youhua Wang

    2. Medical School of Nantong University, 19 Qi-Xiu Road, Nantong, 226001, Jiangsu Province, People’s Republic of China

      Xudong Zhu

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    1. Weidong Zhang
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    Correspondence to Youhua Wang.

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    Weidong Zhang and Xudong Zhu contributed equally to this work.

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    Zhang, W., Zhu, X., Liu, Y. et al. Nur77 Was Essential for Neurite Outgrowth and Involved in Schwann Cell Differentiation After Sciatic Nerve Injury. J Mol Neurosci 57, 38–47 (2015). https://doi.org/10.1007/s12031-015-0575-9

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    • DOI: https://doi.org/10.1007/s12031-015-0575-9

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