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Induction of Human Umbilical Wharton’s Jelly-Derived Stem Cells Toward Oligodendrocyte Phenotype

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Abstract

In this study, we examined phenotypic features of human Wharton’s jelly mesenchymal stem cells-derived oligodendrocytes. We induced human WJMSCs to form OLs by signaling molecules including basic fibroblastic growth factor, platelet-derived growth factor-AA, and triiodothyronine hormone. Differentiated WJMSCs showed morphologic characteristics of an OL phenotype. Expression of surface markers and genes in oligodendrocyte precursor cells or OLs were analyzed by immunocytochemistry staining and real-time polymerase chain reaction, respectively. These results suggest that WJMSCs could be programmed to OLs and might provide a potential source for cell therapy in neurodegenerative disease.

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Acknowledgements

We thank Iran National Science Foundation (INSF) for financial supports and Cellular & Molecular Research Center of Iran University of Medical Sciences for providing us facilities.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Elmira Mikaeili Agah & Kazem Parivar

  2. Iran National Science Foundation (INSF), Tehran, Iran

    Kazem Parivar

  3. Department of Cell and Molecular Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran

    Mohammad Nabiuni

  4. Department of Genetics, Tehran Medical Branch, Islamic Azad University, Tehran, Iran

    Mehrdad Hashemi

  5. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran

    Masoud Soleimani

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  1. Elmira Mikaeili Agah
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  2. Kazem Parivar
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  3. Mohammad Nabiuni
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  4. Mehrdad Hashemi
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  5. Masoud Soleimani
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Correspondence to Elmira Mikaeili Agah.

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Mikaeili Agah, E., Parivar, K., Nabiuni, M. et al. Induction of Human Umbilical Wharton’s Jelly-Derived Stem Cells Toward Oligodendrocyte Phenotype. J Mol Neurosci 51, 328–336 (2013). https://doi.org/10.1007/s12031-013-0094-5

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  • DOI: https://doi.org/10.1007/s12031-013-0094-5

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