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Cellular Uptake and In Vitro Drug Release Studies on Paclitaxel-Loaded Poly(caprolactone)-Grafted Dextran Copolymeric Nanoparticles

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NanoBiotechnology

Abstract

Biodegradable and biocompatible polymers play a key role to provide a solution for sustained chemotherapy, when engineered to nanostructure. One such effort has been put forward to engineer self-assembled poly(caprolactone)-grafted dextran (PGD) core–shell micellar vehicle for anticancer drug (paclitaxel) and presented in this study. Paclitaxel-loaded PGD nanoparticles (NPs) were prepared by a modified oil/water emulsion method and characterized by laser light scattering, atomic force microscopy, and zeta potential measurements. The effects of the copolymeric compositions of PGD NPs on drug encapsulation efficiency, in vitro drug release, cellular uptake, and cell viability of NP formulation with paclitaxel were investigated. The drug encapsulation efficiency was determined spectrophotometrically, and in vitro drug release was estimated using dialysis bag. Human gastric cancer cell line (SNU-638) were used to image and measure the cellular uptake of fluorescent PGD NPs. Cancer cell viability of the drug-loaded PGD NPs was measured by crystal violet staining method. From the results obtained on various aspects, we inferred that the above formulated drug-loaded PGD NPs have significant drug encapsulation efficiency, cellular uptake, and the cancer cell mortality.

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Acknowledgments

This work was supported by the grant of the Korean Ministry of Education, Science and Technology (The Regional Core Research Program/Center for Health Care Technology Development) and Korean Government Project no.: 10028211 through Ministry of Commerce, Industry and Energy Department. Author is thankful to Mr. Jong Bae Park of KBSI, Jeonju branch, for utilizing his skill to do AFM investigations.

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Authors and Affiliations

  1. Center for Healthcare Technology Development, Chonbuk National University, Jeonju, 561-756, South Korea

    P. Prabu

  2. College of Veterinary Medicine, Chonbuk National University, Jeonju, 561-756, South Korea

    Atul A. Chaudhari & S. Y. Park

  3. Department of Bionanosystem Engineering, Chonbuk National University, Jeonju, 561-756, South Korea

    J. A. Ko

  4. Department of Chemistry, Bharathiar University, Coimbatore, 641 046, India

    N. Dharmaraj

  5. Department of Textile Engineering, Chonbuk National University, Jeonju, 561-756, South Korea

    H. Y. Kim & M. S. Khil

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  1. P. Prabu
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  2. Atul A. Chaudhari
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  3. J. A. Ko
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  4. N. Dharmaraj
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  5. S. Y. Park
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  7. M. S. Khil
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Correspondence to H. Y. Kim or M. S. Khil.

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Prabu, P., Chaudhari, A.A., Ko, J.A. et al. Cellular Uptake and In Vitro Drug Release Studies on Paclitaxel-Loaded Poly(caprolactone)-Grafted Dextran Copolymeric Nanoparticles. Nanobiotechnol 5, 42–49 (2009). https://doi.org/10.1007/s12030-010-9031-7

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  • DOI: https://doi.org/10.1007/s12030-010-9031-7

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