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Nanoneedle Surface Modification with 2-Methacryloyloxyethyl Phosphorylcholine Polymer to Reduce Nonspecific Protein Adsorption in a Living Cell

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NanoBiotechnology

Abstract

Previously, we developed a new molecular delivery system to target single living cells by using atomic force microscope and ultrathin needle referred to as nanoneedle. This system delivers molecules into the cell by attaching them to the surface of nanoneedle. However, nonspecific protein adsorption on the nanoneedle surface inside the living cells limits the range of application of this system. In the present study, we focused on nonspecific protein adsorption onto the nanoneedle surface inside the cells and examined whether this protein adsorption was reduced by modifying the nanoneedle surface with a biocompatible phospholipid polymer containing 2-methacryloyloxyethyl phosphorylcholine (MPC) unit. MPC polymer coating of the surface of silicon wafer reduced nonspecific adsorption of proteins from liver extracts and prevented the formation of clot-like protein aggregates. MPC polymer also decreased nonspecific adsorption of cytosolic protein onto the nanoneedle surface inside the living cell. On the other hand, MPC polymer showed no effect on nonspecific mechanical interaction between nanoneedle and the cell components. Surface modification with MPC polymer is a useful technique to modify the surface properties of nanoneedle.

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Acknowledgment

This study was partially supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Research and Development in a New Converting Field Based on Nanotechnology and Materials Sciences to JM and 19770163 to TK).

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Authors and Affiliations

  1. The Center for NanoBio Integration, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan

    Takanori Kihara, Kazuhiko Ishihara & Jun Miyake

  2. Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan

    Takanori Kihara, Narutoshi Yoshida, Kazuhiko Ishihara & Jun Miyake

  3. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan

    Shingo Mieda, Chikashi Nakamura & Jun Miyake

  4. Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan

    Kyoko Fukazawa & Kazuhiko Ishihara

  5. Research Institute of Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), 2-41-6 Aomi, Koto-ku, Tokyo, 135-0064, Japan

    Chikashi Nakamura & Jun Miyake

Authors
  1. Takanori Kihara
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  2. Narutoshi Yoshida
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  3. Shingo Mieda
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  4. Kyoko Fukazawa
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  5. Chikashi Nakamura
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  6. Kazuhiko Ishihara
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  7. Jun Miyake
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Corresponding author

Correspondence to Jun Miyake.

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Kihara, T., Yoshida, N., Mieda, S. et al. Nanoneedle Surface Modification with 2-Methacryloyloxyethyl Phosphorylcholine Polymer to Reduce Nonspecific Protein Adsorption in a Living Cell. Nanobiotechnol 3, 127–134 (2007). https://doi.org/10.1007/s12030-008-9002-4

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  • DOI: https://doi.org/10.1007/s12030-008-9002-4

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