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Role of immune tolerance in BALB/c mice with anaphylactic shock after Echinococcus granulosus infection

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Abstract

This study tested the hypothesis that immune tolerance mediated by regulatory T (Treg) cells is protective against cystic echinococcosis (CE)-induced anaphylactic shock. BALB/c mice were inoculated with protoscoleces of Echinococcus granulosus. After 3 months, the presence of cysts in the peritoneal cavity was confirmed after which a subset of mice was sensitized using a cyst fluid suspension to induce anaphylactic shock. While IgE levels were significantly higher in both groups inoculated with E. granulosus as compared to the healthy control group (both P < 0.01), sensitized mice had higher IgE levels as compared with those with E. granulosus alone (P < 0.05). Mice inoculated with E. granulosus alone and sensitized mice both had significantly higher histamine levels as compared to the healthy controls. The proportion of CD4+CD25+Foxp3+ Treg cells relative to CD4+ cells was significantly higher in mice inoculated with E. granulosus alone (P < 0.0167); significantly higher interleukin-10 (IL-10) and tumor growth factor-β (TGF-β1) levels were also noted in this group (all P < 0.01). In contrast, IL-13 and IL-17A levels were significantly higher in the sensitized mice (both P < 0.05). Taken together, these data suggest that the biphasic changes in Treg cell and cytokine levels may be associated with anaphylactic shock induced by CE.

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Acknowledgments

This study was funded by grants from the National Natural Science Foundation of China (No. 81460309) and Research Award Fund of the First Affiliated Hospital of Xinjiang Medical University (No. 2013ZRQN43).

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  1. Department of Anaesthesiology, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Li yu Shan Street, Ürümqi, 830011, China

    Qin Zhang, Jian-Rong Ye, Hai-Mei Ma, Jian-Jiang Wu, Tao Jiang & Hong Zheng

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Correspondence to Hong Zheng.

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Zhang, Q., Ye, JR., Ma, HM. et al. Role of immune tolerance in BALB/c mice with anaphylactic shock after Echinococcus granulosus infection. Immunol Res 64, 233–241 (2016). https://doi.org/10.1007/s12026-015-8741-2

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