Forensic Science, Medicine, and Pathology

, Volume 10, Issue 3, pp 395–400

Can early myocardial infarction-related deaths be diagnosed using postmortem urotensin receptor expression levels?

  • Mustafa Talip Sener
  • Emre Karakus
  • Zekai Halici
  • Erol Akpinar
  • Atilla Topcu
  • Ahmet Nezih Kok
Original Article

DOI: 10.1007/s12024-014-9575-2

Cite this article as:
Sener, M.T., Karakus, E., Halici, Z. et al. Forensic Sci Med Pathol (2014) 10: 395. doi:10.1007/s12024-014-9575-2
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Abstract

Purpose

Myocardial infarction (MI) is one of the most prevalent causes of sudden adult death. It is difficult to diagnose early MI postmortem because there are no typical or characteristic changes in morphology. In this study, changes in the level of the mRNA for the urotensin receptor (UR) were investigated postmortem to determine the suitability of UR as a biomarker for diagnosis of early MI after death.

Methods

An MI rat model was developed by injecting rats with isoproterenol (ISO) (lethal dose 850 mg/kg) or normal saline (control group). The hearts of rats in the control and ISO-induced MI groups were harvested at 0, 1, 3, 6, 12, 24, 48, and 72 h (h) postmortem. The hearts were then immediately submerged in 1 mL of RNA stabilization solution and stored at 4 °C for <1 week before RNA extraction. Relative UR expression analysis was performed using the StepOne Plus Real Time PCR System with cDNA synthesized from rat heart.

Results

Postmortem UR mRNA expression was higher in the ISO-induced MI group than in the control group, at both 4 and 20 °C, at all of the time points examined except 72 h postmortem (p < 0.0001). The largest increases were observed at ambient temperature and 6 h postmortem.

Conclusions

Based on our findings, increased postmortem UR expression could serve as a biomarker to aid diagnosis of early MI.

Keywords

Myocardial infarctionUrotensin receptorSudden deathRats

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Mustafa Talip Sener
    • 1
  • Emre Karakus
    • 2
  • Zekai Halici
    • 3
  • Erol Akpinar
    • 3
  • Atilla Topcu
    • 3
  • Ahmet Nezih Kok
    • 1
  1. 1.Department of Forensic MedicineAtaturk University School of MedicineErzurumTurkey
  2. 2.Department of Pharmacology and ToxicologyAtaturk University School of VeterinaryErzurumTurkey
  3. 3.Department of PharmacologyAtaturk University School of MedicineErzurumTurkey