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Treadmill exercise promotes interleukin 15 expression in skeletal muscle and interleukin 15 receptor alpha expression in adipose tissue of high-fat diet rats

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Abstract

Interleukin 15 (IL-15) has recently been proposed as a myokine involved in regulating lipid metabolism. We investigated the effect of exercise training on IL-15 content in skeletal muscle and expression of IL-15 receptor (IL-15R) in adipose tissue of obese rats. After 12 weeks of a high-fat diet, obese rats underwent treadmill running at 26 m/min (60 min each, 5 days/week for 8 weeks). High-fat diet induced obesity, with increased body weight, body fat, and lipid profile. The level of IL-15 immunoreactivity (IL-15-ir) in plasma and gastrocnemius muscle was lower in obese than control rats, and the mRNA level of IL-15 in gastrocnemius muscle was markedly decreased. The mRNA and protein levels of IL-15R in adipose tissue were markedly lower in obese rats. Compared with sedentary obese rats, treadmill running showed decreased body weight and elevated mRNA expression of IL-15 in muscle and elevated IL-15-ir level in plasma and muscle. The mRNA and protein level of IL-15R were increased in adipose tissue in treadmill running obese rats. Our results showed that exercise training improve obesity and reversed the downregulation of the IL-15 in muscle and IL-15R in adipose tissue induced by high-fat diet.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant no. 81270921), and Beijing Natural Science Foundation (Effects and mechanisms of aerobic exercise on active-peptide secreted by skeletal muscle of rat with insulin resistance), and the Fundamental Research Funds for the Central Universities.

Ethical standards

All animal care and experimental protocols complied with the Animal Management Rules (document No. 55, 2001) of the Ministry of Health of the People’s Republic of China and the guide for the Care and Use of the Laboratory Animals of Peking University Health Science Center.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. School of P.E. and Sports Science, Beijing Normal University, Beijing, 100875, China

    Hongtao Yang, Bo Qu & Jing Zhang

  2. Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University Health Science Center, Beijing, 100191, China

    Jinrui Chang, Lei Zhao, Chaoshu Tang & Yongfen Qi

  3. Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China

    Wenjia Chen

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  1. Hongtao Yang
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Correspondence to Jing Zhang.

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Yang, H., Chang, J., Chen, W. et al. Treadmill exercise promotes interleukin 15 expression in skeletal muscle and interleukin 15 receptor alpha expression in adipose tissue of high-fat diet rats. Endocrine 43, 579–585 (2013). https://doi.org/10.1007/s12020-012-9809-6

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  • DOI: https://doi.org/10.1007/s12020-012-9809-6

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