Stem Cell Reviews and Reports

, Volume 13, Issue 2, pp 258–266

Expression of Desmoglein 2, Desmocollin 3 and Plakophilin 2 in Placenta and Bone Marrow-Derived Mesenchymal Stromal Cells

  • Melanie L. Hart
  • Elisa Rusch
  • Marvin Kaupp
  • Kay Nieselt
  • Wilhelm K. Aicher
Article

DOI: 10.1007/s12015-016-9710-4

Cite this article as:
Hart, M.L., Rusch, E., Kaupp, M. et al. Stem Cell Rev and Rep (2017) 13: 258. doi:10.1007/s12015-016-9710-4

Abstract

Many controversial results exist when comparing mesenchymal stromal cells (MSCs) derived from different sources. Reasons include not only variables in tissue origin, but also methods of cell preparation or choice of expansion media which can strongly influence the expression and hence, function of the cells. In this short report we aimed to investigate the expression of the cell anchoring proteins desmoglein 2, desmocollin 3 and plakophilin 2 in early passage placenta-derived MSCs of fetal (fetal pMSCs) and maternal (maternal pMSCs) origins versus adult bone marrow-derived MSCs (bmMSCs) that were expanded and cultured under the same good manufacturing practice (GMP) conditions. Comprehensive gene expression microarray analysis profiling indicated differential expression of these genes in the different MSC-derived types with fetal pMSCs expressing the highest levels of PKP2, DSC3 and DSG2, followed by maternal pMSCs, while bmMSCs expressed the lowest levels. A higher expression of PKP2 and DSC3 genes in fetal pMSCs was confirmed by qRT-PCR suggesting neonatal increases in the expression of these desmosomal genes vs. adult MSCs. Intracellular desmocollin 3 and desmoglein 2 expression was observed by flow cytometry and cytoplasmic plakophilin 2 by immunofluorescence in all three MSC sources. These data suggest that fetal pMSCs, maternal pMSCs and bmMSCs may anchor intermediate filaments to the plasma membrane via desmocollin 3, desmoglein 2 and plakophilin 2.

Keywords

Desmosomes Mesenchymal stem cells Mesenchymal stromal cells MSC Desmoglein 2 Desmocollin 3 Plakophilin 2 Placenta Bone marrow 

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Melanie L. Hart
    • 1
  • Elisa Rusch
    • 2
  • Marvin Kaupp
    • 2
  • Kay Nieselt
    • 3
  • Wilhelm K. Aicher
    • 2
  1. 1.Laboratory for Cell & Tissue Engineering, Department of Orthopedics and Trauma SurgeryMedical Center - Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of FreiburgFreiburg im BreisgauGermany
  2. 2.Clinical Research Group KFO 273, Department of UrologyUniversity of Tubingen HospitalTubingenGermany
  3. 3.Integrative Transcriptomics, Center for BioinformaticsUniversity of TübingenTübingenGermany

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