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Platelet-Rich Plasma (PRP) Promotes Fetal Mesenchymal Stem/Stromal Cell Migration and Wound Healing Process

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Abstract

Numerous studies have shown the presence of high levels of growth factors during the process of healing. Growth factors act by binding to the cell surface receptors and contribute to the subsequent activation of signal transduction mechanisms. Wound healing requires a complex of biological and molecular events that includes attraction and proliferation of different type of cells to the wound site, differentiation and angiogenesis. More specifically, migration of various cell types, such as endothelial cells and their precursors, mesenchymal stem/stromal cells (MSCs) or skin fibroblasts (DFs) plays an important role in the healing process. In recent years, the application of platelet rich plasma (PRP) to surgical wounds and skin ulcerations is becoming more frequent, as it is believed to accelerate the healing process. The local enrichment of growth factors at the wound after PRP application causes a stimulation of tissue regeneration. Herein, we studied: (i) the effect of autologous PRP in skin ulcers of patients of different aetiology, (ii) the proteomic profile of PRP, (iii) the migration potential of amniotic fluid MSCs and DFs in the presence of PRP extract in vitro, (iv) the use of the PRP extract as a substitute for serum in cultivating AF-MSCs. Considering its easy access, PRP may provide a valuable tool in multiple therapeutic approaches.

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Acknowledgments

This research was supported by Grant PENED No. 03ED 652 from the Greek Secretariat of Research and Technology and the European Union and by Grant Greek national funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework Research Funding Program: Heracleitus II, Investing in Knowledge Society through the European Social Fund. We are grateful to Professor Suzanne M. Watt (NDCLS, University of Oxford) for providing DF samples.

Disclosure of Potential Conflicts of Interest

The authors indicate no potential conflicts of interest.

Authorship Contribution

M.G. Roubelakis: Conception and design, experimental procedures, data analysis, data approval and manuscript writing.

O. Trohatou: Experimental procedures, data analysis and manuscript reviewing

A. Roubelakis: PRP samples provision, patient treatment and surveillance, data analysis and manuscript reviewing.

E. Mili: PRP collection, patient treatment, experimental procedures, data analysis

I. Kalaitzopoulos: PRP samples provision, patient treatment and surveillance

Georgios Papazoglou: Patient treatment and surveillance

K.I. Pappa: Amniotic fluid samples provision

N.P. Anagnou: Financial support and manuscript reviewing

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Authors and Affiliations

  1. Laboratory of Biology, University of Athens, School of Medicine, Michalakopoulou 176, Athens, 115 27, Greece

    Maria G. Roubelakis, Ourania Trohatou, Evgenia Mili & Nicholas P. Anagnou

  2. Cell and Gene Therapy Laboratory, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens (BRFAA), Athens, Greece

    Maria G. Roubelakis, Ourania Trohatou & Nicholas P. Anagnou

  3. Department of Cardiothoracic Surgery, Southampton University Hospitals, Southampton, UK

    Apostolos Roubelakis

  4. Department of Surgery, Amalia Fleming Hospital, Athens, Greece

    Apostolos Roubelakis, Ioannis Kalaitzopoulos & Georgios Papazoglou

  5. Microbial and Biotechnology Unity, Department of Biology, University of Athens, Athens, Greece

    Evgenia Mili

  6. First Department of Obstetrics and Gynecology, University of Athens School of Medicine, Athens, Greece

    Κalliopi I. Pappa

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  1. Maria G. Roubelakis
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  2. Ourania Trohatou
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  3. Apostolos Roubelakis
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  8. Nicholas P. Anagnou
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Correspondence to Maria G. Roubelakis.

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Roubelakis, M.G., Trohatou, O., Roubelakis, A. et al. Platelet-Rich Plasma (PRP) Promotes Fetal Mesenchymal Stem/Stromal Cell Migration and Wound Healing Process. Stem Cell Rev and Rep 10, 417–428 (2014). https://doi.org/10.1007/s12015-013-9494-8

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