Article

Stem Cell Reviews and Reports

, Volume 9, Issue 2, pp 148-157

Thermoresponsive Substrates used for the Expansion of Human Mesenchymal Stem Cells and the Preservation of Immunophenotype

  • Maria E. NashAffiliated withDepartment of Applied Macromolecular Chemistry, Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSICSchool of Chemistry, National University of Ireland Email author 
  • , Xingliang FanAffiliated withNational Centre for Biomedical Engineering Science, National University of Ireland
  • , William M. CarrollAffiliated withSchool of Chemistry, National University of Ireland
  • , Alexander V. GorelovAffiliated withSchool of Chemistry and Chemical Biology, University College Dublin
  • , Frank P. BarryAffiliated withRegenerative Medicine Institute, National University of Ireland Galway
  • , Georgina ShawAffiliated withRegenerative Medicine Institute, National University of Ireland Galway
  • , Yury A. RochevAffiliated withSchool of Chemistry, National University of IrelandNational Centre for Biomedical Engineering Science, National University of Ireland

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Abstract

The facile regeneration of undifferentiated human mesenchymal stem cells (hMSCs) from thermoresponsive surfaces facilitates the collection of stem cells avoiding the use of animal derived cell detachment agents commonly used in cell culture. This communication proposes a procedure to fabricate coatings from commercially available pNIPAm which is both affordable and a significant simplification on alternative approaches used elsewhere. Solvent casting was used to produce films in the micrometer range and successful cell adhesion and proliferation was highly dependent on the thickness of the coating produced with 1 μm thick coatings supporting cells to confluence. 3T3 cell sheets and hMSCs were successfully detached from the cast coatings upon temperature reduction. Furthermore, results indicate that the hMSCs remained undifferentiated as the surface receptor profile of hMSCs was not altered when cells were detached in this manner.

Keywords

Thermoresponsive polymers Human mesenchymal stem cells Cell expansion and regeneration Flow cytometry Stem cell differentiation