, Volume 7, Issue 3, pp 693-702,
Open Access This content is freely available online to anyone, anywhere at any time.
Date: 24 Feb 2011

Induced Pluripotent Stem Cell Lines Derived from Equine Fibroblasts

Abstract

The domesticated horse represents substantial value for the related sports and recreational fields, and holds enormous potential as a model for a range of medical conditions commonly found in humans. Most notable of these are injuries to muscles, tendons, ligaments and joints. Induced pluripotent stem (iPS) cells have sparked tremendous hopes for future regenerative therapies of conditions that today are not possible to cure. Equine iPS (EiPS) cells, in addition to bringing promises to the veterinary field, open up the opportunity to utilize horses for the validation of stem cell based therapies before moving into the human clinical setting. In this study, we report the generation of iPS cells from equine fibroblasts using a piggyBac (PB) transposon-based method to deliver transgenes containing the reprogramming factors Oct4, Sox2, Klf4 and c-Myc, expressed in a temporally regulated fashion. The established iPS cell lines express hallmark pluripotency markers, display a stable karyotype even during long-term culture, and readily form complex teratomas containing all three embryonic germ layer derived tissues upon in vivo grafting into immunocompromised mice. Our EiPS cell lines hold the promise to enable the development of a whole new range of stem cell-based regenerative therapies in veterinary medicine, as well as aid the development of preclinical models for human applications. EiPS cell could also potentially be used to revive recently extinct or currently threatened equine species.

Kristina Nagy: Conception and design, collection and assembly of data, data analysis and interpretation, manuscript writing
Hoon-Ki Sung: Collection and assembly of data, data analysis and interpretation
Puzheng Zang: Collection and assembly of data
Simon Laflamme: Collection and assembly of data
Patrick Vincent: Collection and assembly of data
Siamak Agha-Mohammadi: Provision of study material
Knut Woltjen: Provision of study material, conception and design
Claudio Monetti: Collection and assembly of data
Iacovos P. Michael: Collection and assembly of data
Lawrence C. Smith: Conception and design, provision of study materials, data analysis and interpretation
Andras Nagy: Conception and design, data analysis and interpretation, final approval of manuscript
This work was supported by funds from the Canadian Stem Cell Network and the Canadian Research Chair in Stem Cells and Regeneration (AN) and the Canadian Research Chair in Animal Cloning and Stem Cells (LCS).
An erratum to this article can be found at http://dx.doi.org/10.1007/s12015-011-9309-8