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Anti- and Pro-apoptotic Bcl2 Proteins Distribution and Metabolic Profile in Human Coronary Aorta Endothelial Cells Before and After HypPDT

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Abstract

Understanding apoptosis regulatory mechanisms in endothelial cells (ECs) has great importance for the development of novel therapy strategies for cancer and cardiovascular pathologies. An oxidative stress with the generation of reactive oxygen species (ROS) is a common mechanism causing ECs’ dysfunction and apoptosis. The generation of ROS can be triggered by various stimuli including photodynamic therapy (PDT). In most PDT treatments, photosensitizer (PS) is administered systemically, and thus, possibility of high exposure to PS in the ECs remains high. PS accumulation in ECs may be clinically relevant even without PDT, if PS molecules affect the pro-apoptotic cascade without illumination. In the present work, we focused on Hypericin (Hyp) and HypPDT effects on the cell viability, oxidative stress, and the distribution of Bcl2 family members in human coronary artery endothelial (HCAEC) cells. Our findings show that the presence of Hyp itself has an effect on cell viability, oxidative stress, and the distribution of Bcl2 family members, without affecting the mitochondria function. In contrast, HypPDT resulted in mitochondria dysfunction, further increase of oxidative stress and effect on the distribution of Bcl2 family members, and in primarily necrotic type of death in HCAEC cells.

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Acknowledgments

Supported by the EU 7FP grant PIRG06-GA-2009-256580 and by EU Structural Fund ITMS26110230013; by the Slovak Grant Agency VEGA -1-0111-12 and VEGA 1/0425/15; and by the Slovak Research and Development Agency APVV-0134-11.

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Authors and Affiliations

  1. Department of Biophysics, Faculty of Natural Sciences, PJ Safarik University, Jesenna 5, Kosice, Slovakia

    Mária Maslaňáková, Lucia Balogová, Pavol Miškovský & Katarína Štroffeková

  2. Center of Interdisciplinary Biosciences, Faculty of Natural Sciences, PJ Safarik University, Kosice, Slovakia

    Pavol Miškovský

  3. Department of Respiratory Medicine, Faculty of Medicine, P.J. Safarik University, Kosice, Slovakia

    Ružena Tkáčová

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  1. Mária Maslaňáková
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  2. Lucia Balogová
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Correspondence to Katarína Štroffeková.

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Maslaňáková, M., Balogová, L., Miškovský, P. et al. Anti- and Pro-apoptotic Bcl2 Proteins Distribution and Metabolic Profile in Human Coronary Aorta Endothelial Cells Before and After HypPDT. Cell Biochem Biophys 74, 435–447 (2016). https://doi.org/10.1007/s12013-016-0740-y

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