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Association of an Exon SNP of SLC2A9 Gene with Hyperuricemia Complicated with Type 2 Diabetes Mellitus in the Chinese Male Han Population

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Abstract

Several recent genome-wide association studies and following studies have identified that genetic variants of SLC2A9 are associated with hyperuricemia (HUA) and diabetes mellitus (DM). Here, we set to investigate whether the exon 9 of SLC2A9 gene variations is associated with HUA complicated with Type 2 DM (T2DM) in the Chinese male Han population. The present study was designed to study rs2280205 polymorphism in exon 9 of SLC2A9 in 232 Chinese male subjects. Rs2280205 locus was genotyped in 52 T2DM subjects, 65 HUA subjects, 55 subjects with HUA complicated with T2DM, as well as 60 control subjects in this study. DNA from peripheral blood was purified and amplified by polymerase chain reaction (PCR). The PCR products were then digested by restriction enzyme MSPI, and part of PCR products was sequenced and analyzed. There was no significant difference in the levels of cholesterol, creatinine, and urea nitrogen between the Control Group and the HUA group. There was also no significant difference in levels of cholesterol between the DM group and Control Group. No significant difference in cholesterol and uric acid was observed between the HUA group and the HUA accompanied with DM group (P > 0.05). However, there was no statistical significance in the genotype frequency in these groups (P > 0.01). Results of the present study suggest that the exon 9 of SLC2A9 gene 109C/T polymorphism is not associated with HUA and diabetes in population living in the coastal area of Shandong province, China.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (81100554), Young and Middle-Aged Scientists Research Awards Fund of Shandong Province (BS2012YY003), the Scientific and Technical Development Project of Department of health of Shandong Province (2011QZ007), a Project of Shandong Province Higher Educational Science and Technology Program (J14LK11), and the Scientific and Technical Development Project of Qingdao (12-1-4-20-jc, 2012-1-3-2-(1)-nsh, and 2013-13-008-YY).

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The authors declare no financial conflict of interest.

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Authors and Affiliations

  1. The Affiliated Hospital of Qingdao University, Qingdao, 266003, People’s Republic of China.

    Shi-Chao Xing, Xu-Fu Wang, Zhi-Min Miao, Xue-Zhi Zhang & Ying Yuan

  2. Key Laboratory of Metabolic Diseases, Qingdao, Shandong Province, People’s Republic of China.

    Shi-Chao Xing

  3. The People’s Hospital of Linqu County, Weifang, Shandong Province, People’s Republic of China.

    Jun Zheng

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  1. Shi-Chao Xing
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  2. Xu-Fu Wang
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  3. Zhi-Min Miao
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  4. Xue-Zhi Zhang
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  5. Jun Zheng
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  6. Ying Yuan
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Correspondence to Shi-Chao Xing or Ying Yuan.

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Xing, SC., Wang, XF., Miao, ZM. et al. Association of an Exon SNP of SLC2A9 Gene with Hyperuricemia Complicated with Type 2 Diabetes Mellitus in the Chinese Male Han Population. Cell Biochem Biophys 71, 1335–1339 (2015). https://doi.org/10.1007/s12013-014-0353-2

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  • DOI: https://doi.org/10.1007/s12013-014-0353-2

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