Abstract
This study evaluated the antioxidative and cardioprotective effects of total flavonoids extracted from Xinjiang sprig Rosa rugosa on ischemia/reperfusion (I/R) injury using an isolated Langendorff rat heart model. The possible mechanism of Xinjiang sprig rose total flavonoid (XSRTF) against I/R injury was also studied. XSRTF (5, 10, and 20 µg/mL) dissolved in Krebs–Henseleit buffer was administered to isolated rat heart. The XSRTF showed remarkable scavenging effects against 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, and superoxide anion radicals in vitro. XSRTF pretreatment improved the heart rate, increased LVDP, and decreased CK and LDH levels in coronary flow. This pretreatment also increased SOD activity and GSH/GSSG ratio but decreased MDA, TNF-α, and CRP levels and IL-8 and IL-6 activities. The infarct size and cell apoptosis in the hearts from the XSRTF-treated group were lower than those in the hearts from the I/R group. Therefore, the cardioprotective effects of XSRTF may be attributed to its antioxidant, antiapoptotic, and anti-inflammatory activities.
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Salie, R., Huisamen, B., & Lochner, A. (2014). High carbohydrate and high fat diets protect the heart against ischaemia/reperfusion injury. Cardiovascular Diabetology, 13(1), 109.
Shahzad, T., Kasseckert, S. A., Iraqi, W., Johnson, V., Schulz, R., Schlüter, K. D., et al. (2013). Mechanisms involved in postconditioning protection of cardiomyocytes against acute reperfusion injury. Journal of Molecular and Cellular Cardiology, 58, 209–216.
Buja, L. M., & Weerasinghe, P. (2010). Unresolved issues in myocardial reperfusion injury. Cardiovascular Pathology, 19(1), 29–35.
Lavie, L. (2014). Oxidative stress in obstructive sleep apnea and intermittent hypoxia—Revisited—The bad ugly and good: Implications to the heart and brain. Sleep Medicine Reviews. doi:10.1016/j.smrv.2014.07.003.
Chan, P. H. (2001). Reactive oxygen radicals in signaling and damage in the ischemic brain. Journal of Cerebral Blood Flow & Metabolism, 21(1), 2–14.
Quan, W., Yin, Y., Xi, M., Zhou, D., Zhu, Y., Guan, Y., et al. (2013). Antioxidant properties of magnesium lithospermate B contribute to the cardioprotection against myocardial ischemia/reperfusion injury in vivo and in vitro. Journal of Traditional Chinese Medicine, 33(1), 85–91.
Yu, L., Sun, Y., Cheng, L., Jin, Z., Yang, Y., Zhai, M., et al. (2014). Melatonin receptor-mediated protection against myocardial ischemia/reperfusion injury: Role of SIRT1. Journal of Pineal Research, 57(2), 228–238.
Pan, Q., Qin, X., Ma, S., Wang, H., Cheng, K., Song, X., et al. (2014). Myocardial protective effect of extracellular superoxide dismutase gene modified bone marrow mesenchymal stromal cells on infarcted mice hearts. Theranostics, 4(5), 475–486.
Xu, X. N., Niu, Z. R., Wang, S. B., Chen, Y. C., Gao, L., Fang, L. H., & Du, G. H. (2014). Effect and mechanism of total flavonoids of bugloss on rats with myocardial ischemia and reperfusion injury. Yao xue xue bao= Acta pharmaceutica sinica, 49(6), 875–881.
Ma, Y., Wang, Y., Gao, Y., Fu, Y., & Li, J. (2014). Total flavonoids from Ganshanbian (Herba Hyperici Attenuati) effect the expression of CaL-alpha1C and K(ATP)-Kir6.1 mRNA of the myocardial cell membrane in myocardial ischemia-reperfusion arrhythmia rats. Journal of Traditional Chinese Medicine, 34(3), 357–361.
Nowak, R., Olech, M., Pecio, L., Oleszek, W., Los, R., Malm, A., & Rzymowska, J. (2014). Cytotoxic, antioxidant, antimicrobial properties and chemical composition of rose petals. Journal of the Science of Food and Agriculture, 94(3), 560–567.
Ozyürek, M., Bektaşoğlu, B., Güçlü, K., & Apak, R. (2008). Hydroxyl radical scavenging assay of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method using catalase for hydrogen peroxide degradation. Analytica Chimica Acta, 616(2), 196–206.
Kolodziejczyk-Czepas, J., Nowak, P., Kowalska, I., & Stochmal, A. (2014). Biological activity of clovers—Free radical scavenging ability and antioxidant action of six Trifolium species. Pharmaceutical Biology, 52(10), 1308–1314.
Dudonné, S., Vitrac, X., Coutière, P., Woillez, M., & Mérillon, J. M. (2009). Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industria interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. Journal of Agricultural and Food Chemistry, 57(5), 1768–1774.
Brito, A., Areche, C., Sepúlveda, B., Kennelly, E. J., & Simirgiotis, M. J. (2014). Anthocyanin characterization, total phenolic quantification and antioxidant features of some Chilean edible berry extracts. Molecules, 19(8), 10936–10955.
Shan, L., Li, J., Wei, M., Ma, J., Wan, L., Zhu, W., et al. (2010). Disruption of Rac1 signaling reduces ischemia-reperfusion injury in the diabetic heart by inhibiting calpain. Free Radical Biology and Medicine, 49(11), 1804–1814.
Han, J., Wang, D., Yu, B., Wang, Y., Ren, H., Zhang, B., Wang, Y., Zheng, Q. (2014). Cardioprotection against ischemia/reperfusion by licochalcone B in isolated rat hearts. Oxidative Medicine and Cellular Longevity. doi:10.1155/2014/134862.
Jiang, J., Yuan, X., Wang, T., Chen, H., Zhao, H., Yan, X., et al. (2014). Antioxidative and cardioprotective effects of total flavonoids extracted from Dracocephalum moldavica L. Against acute schemia/reperfusion-induced myocardial injury in isolated rat heart. Cardiovascular Toxicology, 14(1), 74–82.
Abd-Elsameea, A. A., Moustaf, A. A., & Mohamed, A. M. (2014). Modulation of the oxidative stress by metformin in the cerebrum of rats exposed to global cerebral ischemia and ischemia/reperfusion. European Review of Medicine Pharmacology Science, 18(16), 2387–2392.
Khan, M., Varadharaj, S., Ganesan, L. P., Shobha, J. C., Naidu, M. U., Parinandi, N. L., et al. (2006). C-phycocyanin protects against ischemia-reperfusion injury of heart through involvement of p38 MAPK and ERK signaling. American Journal of Physiology-Heart and Circulatory Physiology, 290(5), H2136–H2145.
AkhlaghM, Bandy B. (2009). Mechanisms of flavonoid protection against myocardial ischemia-reperfusion injury. Journal of Molecular and Cellular Cardiology, 46(3), 309–317.
Cárdenas-Rodríguez, N., González-Trujano, M. E., Aguirre-Hernández, E., Ruíz-García, M., Sampieri, A. 3rd., Coballase-Urrutia, E., Carmona-Aparicio, L. (2014). Anticonvulsant and antioxidant effects of Tilia americana var. Mexicana and flavonoids constituents in the pentylenetetrazole-induced seizures. Oxidative Medicine and Cellular Longevity. doi:10.1155/2014/329172.
Ikizler, M., Erkasap, N., Dernek, S., Kural, T., & Kaygisiz, Z. (2007). Dietary polyphenol quercetin protects rat hearts during reperfusion: enhanced antioxidant capacity with chronictreatment. Anadolu kardiyoloji dergisi: AKD = the Anatolian Journal of Cardiology, 7(4), 404–410.
Hu, N., Dong, M., & Ren, J. (2014). Hydrogen sulfide alleviates cardiac contractile dysfunction in an Akt2-knockout murine model of insulin resistance: role of mitochondria injury and apoptosis. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 306(10), R761–R771.
Oh, Y. B., Ahn, M., Lee, S. M., Koh, H. W., Lee, S. H., Kim, S. H., & Park, B. H. (2013). Inhibition of Janus activated kinase-3 protects against myocardial ischemia and reperfusion injury in mice. Experimental & Molecular Medicine, 45, e23.
Frangogiannis, N. G., Smith, C. W., & Entman, M. L. (2002). The inflammatory response in myocardial infarction. Cardiovascular Research, 53, 31–47.
de Haan, J. J., Smeets, M. B., Pasterkamp, G., & Arslan, F. (2013). Danger signals in the initiation of the inflammatory response after myocardial infarction. Mediators of Inflammation, 2013, 206039.
Han, J., Wang, D., Yu, B., Wang, Y., Ren, H., Zhang, B., et al. (2014). Cardioprotection against ischemia/reperfusion by licochalcone B in isolated rat hearts. Oxidative Medicine and Cellular Longevity, 2014, 134862.
Marfella, R., Cacciapuoti, F., Siniscalchi, M., Sasso, F. C., Marchese, F., Cinone, F., et al. (2006). Effect of moderate red wine intake on cardiac prognosis after recent acute myocardial infarction of subjects with Type 2 diabetes mellitus. Diabetic Medicine, 23(9), 974–981.
Heijnen, B. H., Straatsburg, I. H., Padilla, N. D., Van Mierlo, G. J., Hack, C. E., & Van Gulik, T. M. (2006). Inhibition of classical complement activation attenuates liver ischaemia and reperfusion injury in a rat model. Clinical and Experimental Immunology, 143(1), 15–23.
Watanabe, K., Iwahara, C., Nakayama, H., Iwabuchi, K., Matsukawa, T., Yokoyama, K., et al. (2013). Sevoflurane suppresses tumour necrosis factor-α-induced inflammatory responses in small airway epithelial cells after anoxia/reoxygenation. British Journal of Anaesthesia, 110(4), 637–645.
Acknowledgments
This study was supported by the Xinjiang characteristics of natural medicine modernization and development of key technology research (2012BAI30B01) to Wen Chen, the Xinjiang production and Construction Corps funds for Distinguished Young Scientists (2011CD006) to Qiusheng Zheng.
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The authors declare that they have no financial conflict of interests.
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Xuejiao Hou and Jichun Han have contributed equally to this article.
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Hou, X., Han, J., Yuan, C. et al. Cardioprotective Effects of Total Flavonoids Extracted from Xinjiang Sprig Rosa rugosa against Acute Ischemia/Reperfusion-Induced Myocardial Injury in Isolated Rat Heart. Cardiovasc Toxicol 16, 54–66 (2016). https://doi.org/10.1007/s12012-015-9308-0
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DOI: https://doi.org/10.1007/s12012-015-9308-0